2026-05-17T01:43:56Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/112062024-09-27T08:05:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Garcia-Gonzalez, Irene
author
Mühleder, Severin
author
Fernandez-Chacon, Macarena
author
Benedito, Rui
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
funder
Fundación La Caixa
funder
FWF Austrian Science Fund
2020-10-27T11:53:45Z
2020-10-27T11:53:45Z
2020-10
Front Physiol. 2020; 11:1084
10.3389/fphys.2020.01084
1664-042X
Frontiers in physiology
33071802
http://hdl.handle.net/20.500.12105/11206
Progress in biomedical science is tightly associated with the improvement of methods and genetic tools to manipulate and analyze gene function in mice, the most widely used model organism in biomedical research. The joint effort of numerous individual laboratories and consortiums has contributed to the creation of a large genetic resource that enables scientists to image cells, probe signaling pathways activities, or modify a gene function in any desired cell type or time point, à la carte. However, as these tools significantly increase in number and become more sophisticated, it is more difficult to keep track of each tool's possibilities and understand their advantages and disadvantages. Knowing the best currently available genetic technology to answer a particular biological question is key to reach a higher standard in biomedical research. In this review, we list and discuss the main advantages and disadvantages of available mammalian genetic technology to analyze cardiovascular cell biology at higher cellular and molecular resolution. We start with the most simple and classical genetic approaches and end with the most advanced technology available to fluorescently label cells, conditionally target their genes, image their clonal expansion, and decode their lineages.
eng
Genetic Tools to Study Cardiovascular Biology.
journal article
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URL
https://repisalud.isciii.es/bitstreams/7d40bf27-3b0b-472a-b738-938ad2160c1c/download
File
MD5
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application/pdf
GeneticToolsStudyCardiovascular_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/d0b07b32-71c4-42e5-8a07-5dec17ac7dba/download
File
MD5
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text/plain
GeneticToolsStudyCardiovascular_2020.pdf.txt