2026-05-23T04:19:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/111242024-10-31T11:43:30Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
García-Poyatos, Carolina
author
Cogliati, Sara
author
Calvo, Enrique
author
Hernansanz-Agustin, Pablo
author
Lagarrigue, Sylviane
author
Magni, Ricardo
author
Botos, Marius
author
Langa, Xavier
author
Amati, Francesca
author
Vazquez, Jesus
author
Mercader, Nadia
author
Enriquez, Jose Antonio
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Swiss National Science Foundation
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación La Marató TV3
funder
Fundación La Caixa
funder
Fundación ProCNIC
2020-10-08T12:41:15Z
2020-10-08T12:41:15Z
2020-07-03
EMBO Rep. 2020; 21(7):e50287
10.15252/embr.202050287
1469-3178
EMBO reports
32496654
http://hdl.handle.net/20.500.12105/11124
The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III2 + IV2 , I + IV, and I + III2 + IV2 . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1-/- fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1-/- fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.
eng
Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.
journal article
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