2026-08-29T21:33:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/110662025-04-08T13:12:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Raro, Otávio Hallal Ferreira
author
da Silva, Ravena Maya Cardoso
author
Filho, Edison Moraes Rodrigues
author
Sukiennik, Teresa Cristina Teixeira
author
Stadnik, Claudio
author
Dias, Cícero Armídio Gomes
author
Oteo-Iglesias, Jesus
author
Perez-Vazquez, Maria
funder
Instituto de Salud Carlos III
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2020-09-22T10:41:25Z
2020-09-22T10:41:25Z
2020
Front Microbiol . 2020 Jul 15;11:1563.
10.3389/fmicb.2020.01563
1664-302X
1664-302X
Frontiers in microbiology
32760368
http://hdl.handle.net/20.500.12105/11066
Objectives: Carbapenemase-producing Klebsiella pneumoniae (CP-Kp) is a major cause of infections in transplanted patients and has been associated with high mortality rates in this group. There is a lack of information about the Brazilian structure population of CP-Kp isolated from transplanted patients. By whole-genome sequencing (WGS), we analyzed phylogeny, resistome, virulome of CP-Kp isolates, and the structure of plasmids encoding bla KPC- 2 and bla NDM- 1 genes. Methods: One K. pneumoniae isolated from each selected transplanted patient colonized or infected by CP-Kp over a 16-month period in a hospital complex in Porto Alegre (Brazil) was submitted for WGS. The total number of strains sequenced was 80. The hospital complex in Porto Alegre comprised seven different hospitals. High-resolution SNP typing, core genome multilocus sequence typing (cgMLST), resistance and virulence genes inference, and plasmid reconstruction were performed in 80 CP-Kp. Results: The mortality rate of CP-Kp colonized or infected transplanted inpatients was 21.3% (17/80). Four CP-Kp epidemic clones were described: ST11/KPC-2, ST16/KPC-2, and ST15/NDM-1, all responsible for interhospital outbreaks; and ST437/KPC-2 affecting a single hospital. The average number of acquired resistance and virulence genes was 9 (range = 2-14) and 27 (range = 6-36), respectively. Two plasmids carrying the bla KPC - 2 were constructed and belonged to IncN and IncM types. Additionally, an IncFIB plasmid carrying the bla NDM- 1 was described. Conclusion: We detected intrahospital and interhospital spread of mobile structures and international K. pneumoniae clones as ST11, ST16, and ST15 among transplanted patients, which carry a significant range of acquired resistance and virulence genes and keep spreading across the world.
eng
Transplanted patients
blaKPC−2
blaNDM−1
Whole-genome sequencing
cgMLST
Epidemic clones
Carbapenemase-Producing Klebsiella pneumoniae From Transplanted Patients in Brazil: Phylogeny, Resistome, Virulome and Mobile Genetic Elements Harboring blaKPC-2 or blaNDM-1.
research article
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URL
https://repisalud.isciii.es/bitstreams/003955a6-53e8-42d3-b99a-06da4f87bd28/download
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application/pdf
CarbapenemaseProducingKlebsiellaPneumoniae_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/48b45b67-3118-4121-bb04-0ac09fdfc00d/download
File
MD5
e157aa70b1beb5e28d5195fb64034a22
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text/plain
CarbapenemaseProducingKlebsiellaPneumoniae_2020.pdf.txt