2026-06-14T03:33:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/110612025-06-03T18:12:52Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Álvarez-Alonso, Raquel
author
Zendoia, Ion I
author
Barandika, Jesús Félix
author
Hurtado, Ana
author
López, Ceferino M
author
García-Pérez, Ana L
author
Jado, Isabel
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Basque Government (España)
funder
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
2020-09-22T09:57:20Z
2020-09-22T09:57:20Z
2020
Front Vet Sci . 2020 Jul 10;7:352.
10.3389/fvets.2020.00352
2297-1769
2297-1769
Frontiers in veterinary science
32754620
http://hdl.handle.net/20.500.12105/11061
Progression of Coxiella burnetii infection in four naturally infected sheep flocks, and in their farm environment, was monitored throughout four lambing seasons. Flocks with an active infection were selected based on the presence of C. burnetii DNA in bulk-tank milk (BTM) and a high seroprevalence in yearlings during the previous milking period (Spring 2015). During four consecutive lambing seasons (2015/16-2018/19), samples were collected within 1 week after each lambing period from animals (vaginal swabs, milk and feces from ewes, and yearlings) and the environment (dust indoor sheep premises). BTM samples and aerosols (outdoors and indoors) were monthly collected between lambing and the end of milking. Real-time PCR analyses showed different trends in C. burnetii shedding in the flocks, with a general progressive decrease in bacterial shedding throughout the years, interrupted in three flocks by peaks of reinfection associated with specific management practices. A significant relationship was found between C. burnetii fecal shedding and the bacterial burden detected in dust, whereas shedding by vaginal route affected the detection of C. burnetii in indoor aerosols. Three genotypes were identified: SNP8 (three flocks, 52.9% of the samples), SNP1 (two flocks, 44.8% samples), and SNP5 (one flock, two environmental samples). Coxiella burnetii viability in dust measured by culture in Vero cells was demonstrated in two of the flocks, even during the fourth lambing season. The results showed that infection can remain active for over 5 years if effective control and biosafety measures are not correctly implemented.
eng
Monitoring Coxiella burnetii Infection in Naturally Infected Dairy Sheep Flocks Throughout Four Lambing Seasons and Investigation of Viable Bacteria
research article
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URL
https://repisalud.isciii.es/bitstreams/4ee28654-b5aa-4892-9ab0-0124abe20875/download
File
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application/pdf
MonitoringCoxiellaBurnetiiInfection_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/802ffa45-d9c5-4603-8eaa-6b12515b8b81/download
File
MD5
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text/plain
MonitoringCoxiellaBurnetiiInfection_2020.pdf.txt