2026-08-29T14:05:02Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/109122024-11-30T01:04:49Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Toda, Haruka
author
Diaz-Varela, Miriam
author
Segui-Barber, Joan
author
Roobsoong, Wanlapa
author
Baro, Barbara
author
Garcia-Silva, Susana
author
Galiano, Alicia
author
Gualdrón-López, Melisa
author
Almeida, Anne C G
author
Brito, Marcelo A M
author
de Melo, Gisely Cardoso
author
Aparici-Herraiz, Iris
author
Castro-Cavadía, Carlos
author
Monteiro, Wuelton Marcelo
author
Borràs, Eva
author
Sabidó, Eduard
author
Almeida, Igor C
author
Chojnacki, Jakub
author
Martinez-Picado, Javier
author
Calvo, Maria
author
Armengol, Pilar
author
Carmona-Fonseca, Jaime
author
Yasnot, Maria Fernanda
author
Lauzurica, Ricardo
author
Marcilla, Antonio
author
Galinski, Mary R
author
Lacerda, Marcus V G
author
Sattabongkot, Jetsumon
author
Fernandez-Becerra, Carmen
author
Del Portillo, Hernando A
author
Peinado Selgas, Hector
2020-09-01T10:00:10Z
2020-09-01T10:00:10Z
2020
Nat Commun. 2020 ;11(1):2761.
10.1038/s41467-020-16337-y
2041-1723
Nature communications
32487994
http://hdl.handle.net/20.500.12105/10912
Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. vivax acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) are therefore major components of circulating plasma holding insights into pathological processes. Here, we demonstrate that plasma-derived EVs from Plasmodium vivax patients (PvEVs) are preferentially uptaken by human spleen fibroblasts (hSFs) as compared to the uptake of EVs from healthy individuals. Moreover, this uptake induces specific upregulation of ICAM-1 associated with the translocation of NF-kB to the nucleus. After this uptake, P. vivax-infected reticulocytes obtained from patients show specific adhesion properties to hSFs, reversed by inhibiting NF-kB translocation to the nucleus. Together, these data provide physiological EV-based insights into the mechanisms of human malaria pathology and support the existence of P. vivax-adherent parasite subpopulations in the microvasculature of the human spleen.
eng
Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence.
journal article
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
URL
https://repisalud.isciii.es/bitstreams/35fcceb1-594a-4f3e-9f71-f14f6b8c6972/download
File
MD5
f6473a788edd4155f5f0f21cf13ceb0a
2523412
application/pdf
Plasma-derivedextracellularvesicles_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/d7640753-646f-4aae-86e5-ea9ecc28d32e/download
File
MD5
f7ac53b4512dc8ea31fc335a93a116fa
8750778
application/pdf
41467_2020_16337_MOESM1_ESM.pdf
URL
https://repisalud.isciii.es/bitstreams/678aa839-3741-4c82-a0c5-6e38b26beeb3/download
File
MD5
9cd1116363d8aa5a13a49937da829ecc
2607784
application/pdf
41467_2020_16337_MOESM2_ESM.pdf
URL
https://repisalud.isciii.es/bitstreams/b8035c35-1ebf-4150-8bec-57557b351d9a/download
File
MD5
93c9dbee68cc1d50fd0fb06c577f2c1a
255675
application/pdf
41467_2020_16337_MOESM3_ESM.pdf
URL
https://repisalud.isciii.es/bitstreams/1fda7906-61da-45ba-a05c-27b40b2f7c5c/download
File
MD5
e761c68a7602fa11814f94142e1d816b
15726
application/vnd.openxmlformats-officedocument.wordprocessingml.document
41467_2020_16337_MOESM4_ESM.docx
URL
https://repisalud.isciii.es/bitstreams/be766bdd-46ed-43c5-8cee-76f0063bd752/download
File
MD5
7d495bad2f3fcd41bf997ac97f8c13fb
9969
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Copia de 41467_2020_16337_MOESM5_ESM.xlsx
URL
https://repisalud.isciii.es/bitstreams/f983470d-c1c1-4478-893b-91f914140d4c/download
File
MD5
c73e507402921298be337106ce4acd45
183033
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Copia de 41467_2020_16337_MOESM6_ESM.xlsx
URL
https://repisalud.isciii.es/bitstreams/745cfa7a-1f4f-4516-9d89-bc046f698434/download
File
MD5
38e8757c2de8c51ed4c749842be6d62e
20336
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Copia de 41467_2020_16337_MOESM7_ESM.xlsx
URL
https://repisalud.isciii.es/bitstreams/d0da8d26-6dc0-4e3e-82ef-cb86d5f3e865/download
File
MD5
9a2715c9e46108a2cb74ba9b8b61dc6d
27054
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Copia de 41467_2020_16337_MOESM8_ESM.xlsx
URL
https://repisalud.isciii.es/bitstreams/bbce73f9-f5e7-491d-ad06-dcf3cd23661d/download
File
MD5
01025d9b0eccc3860a2cebf4f3467a32
81440
text/plain
Plasma-derivedextracellularvesicles_2020.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/f0329376-ce82-4318-83fb-f9ece99419ba/download
File
MD5
fe8983ab88ee54f7cfc39047b504f863
8063
text/plain
41467_2020_16337_MOESM1_ESM.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/c04afcee-d08e-4f1b-8da7-4ee1e5e99c1f/download
File
MD5
e6fd5e522fc20926df4b154f41dd8788
53492
text/plain
41467_2020_16337_MOESM2_ESM.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/2f60504c-1f22-49d7-afc5-ba470fc814ee/download
File
MD5
a6decfa26404009435e34f355069fd75
15122
text/plain
41467_2020_16337_MOESM3_ESM.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/083e6847-345f-409a-bab2-19d6d9354016/download
File
MD5
0bede0ca284b55bc08df154c4078c09b
2466
text/plain
41467_2020_16337_MOESM4_ESM.docx.txt
URL
https://repisalud.isciii.es/bitstreams/2cd16ba9-a9a2-4dd4-a453-840046337f66/download
File
MD5
546b223ff24e826e552ce69cf952aabb
767
text/plain
Copia de 41467_2020_16337_MOESM5_ESM.xlsx.txt
URL
https://repisalud.isciii.es/bitstreams/b1a6cfe8-a885-418c-862a-d3573a2dcf20/download
File
MD5
7d9c71a1d3066fc94fb6655288c5d8fa
100002
text/plain
Copia de 41467_2020_16337_MOESM6_ESM.xlsx.txt
URL
https://repisalud.isciii.es/bitstreams/21326a20-5306-4b19-8dbc-32e5325ddaee/download
File
MD5
ff9fe9583840dbedadc82a0f3b0eb80c
9006
text/plain
Copia de 41467_2020_16337_MOESM7_ESM.xlsx.txt
URL
https://repisalud.isciii.es/bitstreams/6ced4f39-ce91-42b7-9414-cfdd1d6cec99/download
File
MD5
1ba01d57b6ece05952e3f6e07e358980
8257
text/plain
Copia de 41467_2020_16337_MOESM8_ESM.xlsx.txt