2026-06-14T14:33:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/108722025-06-12T12:35:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Mekonnen, Gebeyaw G
author
Tedla, Bemnet A
author
Pickering, Darren
author
Becker, Luke
author
Wang, Lei
author
Zhan, Bin
author
Bottazzi, Maria Elena
author
Loukas, Alex
author
Pearson, Mark S
author
Sotillo, Javier
funder
National Health and Medical Research Council (Australia)
2020-08-10T08:31:32Z
2020-08-10T08:31:32Z
2020-07-24
Vaccines (Basel). 2020 Jul 24;8(3):E416.
10.3390/vaccines8030416
2076-393X
Vaccines
32722279
http://hdl.handle.net/20.500.12105/10872
Helminth parasites release extracellular vesicles which interact with the surrounding host tissues, mediating host-parasite communication and other fundamental processes of parasitism. As such, vesicle proteins present attractive targets for the development of novel intervention strategies to control these parasites and the diseases they cause. Herein, we describe the first proteomic analysis by LC-MS/MS of two types of extracellular vesicles (exosome-like, 120 k pellet vesicles and microvesicle-like, 15 k pellet vesicles) from adult Schistosoma haematobium worms. A total of 57 and 330 proteins were identified in the 120 k pellet vesicles and larger 15 k pellet vesicles, respectively, and some of the most abundant molecules included homologues of known helminth vaccine and diagnostic candidates such as Sm-TSP2, Sm23, glutathione S-transferase, saponins and aminopeptidases. Tetraspanins were highly represented in the analysis and found in both vesicle types. Vaccination of mice with recombinant versions of three of these tetraspanins induced protection in a heterologous challenge (S. mansoni) model of infection, resulting in significant reductions (averaged across two independent trials) in liver (47%, 38% and 41%) and intestinal (47%, 45% and 41%) egg burdens. These findings offer insight into the mechanisms by which anti-tetraspanin antibodies confer protection and highlight the potential that extracellular vesicle surface proteins offer as anti-helminth vaccines.
eng
Schistosoma haematobium Extracellular Vesicle Proteins Confer Protection in a Heterologous Model of Schistosomiasis
research article
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URL
https://repisalud.isciii.es/bitstreams/b6071c2a-145b-4eb1-95f0-92725c474ecc/download
File
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SchistosomaHaematobiumExtracellularVesicle_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/307a1853-20b1-4068-bd16-6776b8d7dc06/download
File
MD5
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text/plain
SchistosomaHaematobiumExtracellularVesicle_2020.pdf.txt