2026-06-14T03:33:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/108462025-06-17T08:59:27Zcom_20.500.12105_14556com_20.500.12105_2337com_20.500.12105_2336com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_14558col_20.500.12105_19616
Repisalud
author
Morales-Molina, Alvaro
author
Rodriguez-Milla, Miguel A
author
Gimenez-Sanchez, Alicia
author
Perise-Barrios, Ana Judith
author
Garcia-Castro, Javier
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Fundación Oncohematología Infantil
funder
Asociación Pablo Ugarte contra el cáncer infantil
2020-07-27T20:18:31Z
2020-07-27T20:18:31Z
2020-07-16
Cancers (Basel) . 2020 Jul 16;12(7):E1920.
10.3390/cancers12071920
2072-6694
2072-6694
Cancers
32708639
http://hdl.handle.net/20.500.12105/10846
Oncolytic virotherapy uses viruses designed to selectively replicate in cancer cells. An alternative to intratumoral administration is to use mesenchymal stem cells (MSCs) to transport the oncolytic viruses to the tumor site. Following this strategy, our group has already applied this treatment to children and adults in a human clinical trial and a veterinary trial, with good clinical responses and excellent safety profiles. However, the development of immunocompetent cancer mouse models is still necessary for the study and improvement of oncolytic viroimmunotherapies. Here we have studied the antitumor efficacy, immune response, and mechanism of action of a complete murine version of our cellular virotherapy in mouse models of renal adenocarcinoma and melanoma. We used mouse MSCs infected with the mouse oncolytic adenovirus dlE102 (OAd-MSCs). In both models, treatment with OAd-MSCs significantly reduced tumor volumes by 50% and induced a pro-inflammatory tumor microenvironment. Furthermore, treated mice harboring renal adenocarcinoma and melanoma tumors presented increased infiltration of tumor-associated macrophages (TAMs), natural killer cells, and tumor-infiltrating lymphocytes (TILs). Treated mice also presented lower percentage of TILs expressing programmed cell death protein 1 (PD-1)-the major regulator of T cell exhaustion. In conclusion, treatment with OAd-MSCs significantly reduced tumor volume and induced changes in tumor-infiltrating populations of melanoma and renal cancer.
eng
Oncolytic virus
Adenovirus
MSCs
Immunotherapy
Celyvir
TILs
T cells
PD-1
Renalcancer
Melanoma
Cellular Virotherapy Increases Tumor-Infiltrating Lymphocytes (TIL) and Decreases their PD-1+ Subsets in Mouse Immunocompetent Models
research article
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URL
https://repisalud.isciii.es/bitstreams/e72a65a7-1ef2-492c-a22a-ca0199e2f917/download
File
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application/pdf
CellularVirotherapyIncreasesTumor_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/32e6112f-02cc-4d74-962f-855f2493caa5/download
File
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text/plain
CellularVirotherapyIncreasesTumor_2020.pdf.txt