2026-07-21T14:26:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/107772024-10-31T11:42:05Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Lopez, Daniel
author
Samino, Y
author
Koszinowski, U H
author
Val, Margarita del
funder
Unión Europea
funder
Comunidad de Madrid (España)
funder
Comisión Interministerial de Ciencia y Tecnología (España)
2020-07-15T13:26:37Z
2020-07-15T13:26:37Z
2001-10-15
J Immunol . 2001 Oct 15;167(8):4238-44
10.4049/jimmunol.167.8.4238
0022-1767
Journal of immunology (Baltimore, Md. : 1950)
11591745
http://hdl.handle.net/20.500.12105/10777
CTL recognize peptides that derive from viral protein Ags by proteolytic processing and are presented by MHC class I molecules. In this study we tested whether coexpression of viral Ags in the same cell leads to competition between them. To this end, two L(d)-restricted epitopes derived from HIV-1 envelope gp160 (ENV) and from CMV pp89 phosphoprotein were coexpressed. HIV ENV strain IIIB, but not MN variant, impaired recognition by specific CTL of CMV pp89 epitope 9pp89. Susceptibility to inhibition after ENV coexpression was inversely related to the amount of antigenic 9pp89 peptide processed from different antigenic constructs. In line with it, competition decreased the yield of naturally processed antigenic 9pp89 peptide bound to MHC class I molecules in coinfected cells. Also, point mutants of the presenting MHC class I molecule differed in their competition pattern. Collectively, the data imply that competition operates at the step of MHC-peptide complex assembly or stabilization. We conclude that, although not the rule, in certain combinations there is interference between different Ags expressed in the same cell and presented by the same MHC class I allele. These studies have implications for vaccine development and for understanding immunodominance.
eng
HIV envelope protein inhibits MHC class I presentation of a cytomegalovirus protective epitope.
journal article
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URL
https://repisalud.isciii.es/bitstreams/a71f78cf-d038-463b-8f00-0d59045219bf/download
File
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application/pdf
HIVEnvelopeProteinInhibits_2001..pdf
URL
https://repisalud.isciii.es/bitstreams/af521830-270c-4ea1-9f8d-2093f40baec1/download
File
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text/plain
HIVEnvelopeProteinInhibits_2001..pdf.txt