2026-05-22T00:30:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/107202024-09-27T07:55:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Delgado, Irene author López-Delgado, Alejandra C author Roselló-Díez, Alberto author Giovinazzo, Giovanna author Cadenas, Vanessa author Fernandez-de-Manuel, Laura author Sanchez-Cabo, Fatima author Anderson, Matthew J author Lewandoski, Mark author Torres, Miguel author 2020-06 The positional information theory proposes that a coordinate system provides information to embryonic cells about their position and orientation along a patterning axis. Cells interpret this information to produce the appropriate pattern. During development, morphogens and interpreter transcription factors provide this information. We report a gradient of Meis homeodomain transcription factors along the mouse limb bud proximo-distal (PD) axis antiparallel to and shaped by the inhibitory action of distal fibroblast growth factor (FGF). Elimination of Meis results in premature limb distalization and HoxA expression, proximalization of PD segmental borders, and phocomelia. Our results show that Meis transcription factors interpret FGF signaling to convey positional information along the limb bud PD axis. These findings establish a new model for the generation of PD identities in the vertebrate limb and provide a molecular basis for the interpretation of FGF signal gradients during axial patterning. Sci Adv. 2020; 6(23):eaaz0742 10.1126/sciadv.aaz0742 2375-2548 Science advances 32537491 http://hdl.handle.net/20.500.12105/10720 Proximo-distal positional information encoded by an Fgf-regulated gradient of homeodomain transcription factors in the vertebrate limb.