2026-08-05T16:12:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/107142024-09-27T09:10:27Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Calvo, Enrique
author
Cogliati, Sara
author
Hernansanz-Agustin, Pablo
author
Loureiro-López, Marta
author
Guaras, Adela
author
Casuso, Rafael A.
author
Garcia-Marques, Fernando
author
Acin-Perez, Rebeca
author
Martí-Mateos, Yolanda
author
Silla-Castro, JC.
author
Carro-Alvarellos, Marta
author
Huertas, Jesús R.
author
Vazquez, Jesus
author
Enriquez, Jose Antonio
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Centro de Investigación Biomedica en Red - CIBER
funder
Instituto de Salud Carlos III
funder
Fundación La Marató TV3
funder
Fundación La Caixa
funder
Fundación ProCNIC
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2020-07-08T10:38:28Z
2020-07-08T10:38:28Z
2020-06
Sci Adv. 2020; 6(26):eaba7509
10.1126/sciadv.aba7509
2375-2548
Science Advances
http://hdl.handle.net/20.500.12105/10714
Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III2 + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III2 + IV) and the physiological role of SCs are controversial. Here, we study C57BL/6J mice harboring nonfunctional SCAF1, the full knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent. By combining quantitative data–independent proteomics, 2D Blue native gel electrophoresis, and functional analysis of enriched respirasome fractions, we show that SCAF1 confers structural attachment between III2 and IV within the N-respirasome, increases NADH-dependent respiration, and reduces reactive oxygen species (ROS). Furthermore, the expression of AOX in cells and mice confirms that CI-CIII superassembly segments the CoQ in two pools and modulates CI-NADH oxidative capacity.
eng
Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool
journal article
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URL
https://repisalud.isciii.es/bitstreams/35acf96f-b3b4-4a23-bac4-cff97c1900ad/download
File
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application/pdf
FunctionalRoleOfRespiratorySupercomplexes_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/f0d822cd-a2ef-4cb4-87d0-d0d7fbe69ef3/download
File
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text/plain
FunctionalRoleOfRespiratorySupercomplexes_2020.pdf.txt