2026-04-29T12:23:46Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/106672025-05-08T10:07:55Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616
Repisalud
author
Macia, Angela
author
Ayllon, Veronica
author
Sánchez, Laura
author
Benkaddour-Boumzaouad, Meriem
author
Muñoz-Lopez, Martin
author
Rubio, Alejandro
author
Amador-Cubero, Suyapa
author
Blanco-Jimenez, Eva
author
Menéndez, Pablo
author
Ng, Philip
author
Widmann, Thomas J.
author
Heras, Sara R.
author
Muotri, Alysson R.
author
Goodier, John L.
author
Garcia-Perez, Jose L.
author
Garcia-Castro, Javier
funder
Unión Europea. Comisión Europea. 7 Programa Marco
funder
United States Department of Defense
funder
National Institutes of Health (Estados Unidos)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Howard Hughes Medical Institute
funder
Wellcome Trust
funder
Unión Europea. Comisión Europea. 7 Programa Marco
2020-07-06T15:12:46Z
2020-07-06T15:12:46Z
2017
Genome Res . 2017 Mar;27(3):335-348.
10.1101/gr.206805.116
1549-5469
1088-9051
Genome research
27965292
http://hdl.handle.net/20.500.12105/10667
Half the human genome is made of transposable elements (TEs), whose ongoing activity continues to impact our genome. LINE-1 (or L1) is an autonomous non-LTR retrotransposon in the human genome, comprising 17% of its genomic mass and containing an average of 80-100 active L1s per average genome that provide a source of inter-individual variation. New LINE-1 insertions are thought to accumulate mostly during human embryogenesis. Surprisingly, the activity of L1s can further impact the somatic human brain genome. However, it is currently unknown whether L1 can retrotranspose in other somatic healthy tissues or if L1 mobilization is restricted to neuronal precursor cells (NPCs) in the human brain. Here, we took advantage of an engineered L1 retrotransposition assay to analyze L1 mobilization rates in human mesenchymal (MSCs) and hematopoietic (HSCs) somatic stem cells. Notably, we have observed that L1 expression and engineered retrotransposition is much lower in both MSCs and HSCs when compared to NPCs. Remarkably, we have further demonstrated for the first time that engineered L1s can retrotranspose efficiently in mature nondividing neuronal cells. Thus, these findings suggest that the degree of somatic mosaicism and the impact of L1 retrotransposition in the human brain is likely much higher than previously thought.
eng
Engineered LINE-1 Retrotransposition in Nondividing Human Neurons
research article
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URL
https://repisalud.isciii.es/bitstreams/af098d62-1c6a-43a7-8169-359b47e52623/download
File
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ce39632595f2f92afd19712fef770200
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EngineeredLINE1Retrotransposition_2017.pdf
URL
https://repisalud.isciii.es/bitstreams/1ef05b0e-1d14-4426-8039-839a321788d7/download
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MD5
58a2151232a7b0ad64ef1b66437990e1
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text/plain
EngineeredLINE1Retrotransposition_2017.pdf.txt