2026-04-29T04:10:23Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/106062024-09-27T09:59:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Perdiguero, Eusebio
author
Moiseeva, Victoria
author
Munoz-Canoves, Pura
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Fundación La Caixa
2020-06-29T11:30:00Z
2020-06-29T11:30:00Z
2019-02
Methods Mol Biol. 2019; 2045:13-23
http://hdl.handle.net/20.500.12105/10606
30771188
10.1007/7651_2019_210
1940-6029
Methods in molecular biology (Clifton, N.J.)
The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice.
eng
Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.
journal article
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URL
https://repisalud.isciii.es/bitstreams/b67b00ba-07b4-4fa3-add3-4b860a0c8a70/download
File
MD5
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application/pdf
SimultaneousIsolationStemNiche_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/8c82b1c8-883f-4e23-bc5c-094f43696711/download
File
MD5
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text/plain
SimultaneousIsolationStemNiche_2019.pdf.txt