2026-04-29T04:09:58Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/106062024-09-27T09:59:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Perdiguero, Eusebio author Moiseeva, Victoria author Munoz-Canoves, Pura author 2019-02 The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice. Methods Mol Biol. 2019; 2045:13-23 http://hdl.handle.net/20.500.12105/10606 30771188 10.1007/7651_2019_210 1940-6029 Methods in molecular biology (Clifton, N.J.) Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.