2026-06-14T03:30:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/105302024-11-29T17:26:11Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Usó, Marta
author
Jantus-Lewintre, Eloisa
author
Bremnes, Roy M
author
Calabuig, Silvia
author
Blasco, Ana
author
Pastor, Enrique
author
Borreda, Irene
author
Molina-Pinelo, Sonia
author
Guijarro, Ricardo
author
Martorell, Miguel
author
Forteza, Jerónimo
author
Camps, Carlos
author
Sirera, Rafael
author
Paz Ares, Luis Gonzaga
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Red Temática de Investigación Cooperativa en Cáncer (RTICC) (España)
2020-06-22T15:42:54Z
2020-06-22T15:42:54Z
2016-08-16
Oncotarget. 2016 ;7(33):52849-52861.
10.18632/oncotarget.10811
1949-2553
Oncotarget
27463005
http://hdl.handle.net/20.500.12105/10530
The prognosis of non-small cell lung cancer (NSCLC) remains poor and heterogeneous and new biomarkers are needed. As the immune system plays a pivotal role in cancer, the study of immune-related markers may provide valuable prognostic information of NSCLC. In 122 formalin-fixed, paraffin-embedded tumor tissue samples from early-stage NSCLC, tumor and tumor-near stromal areas were microdissected and gene expression levels of conventional and regulatory T cell markers were assessed by quantitative polymerase chain reaction. Also, the presence of infiltrating CD4+, CD8+, and FOXP3+ cells in tumor samples was assessed by immunohistochemistry. The relative proportion of conventional and regulatory T cells present in the tumor environment was assessed and found to be key to understand the importance that the immune system analysis has in the prognostics of NSCLC patients. The presence of CD8+ cells in the tumor compartment was associated with better outcome, whereas the presence of FOXP3+ cells was associated with worse overall survival. The negative prognostic value of combined biomarkers, indicating high levels of FOXP3 in the stroma and low levels of CD4 or CD8 in tumors, was observed at mRNA level and was validated by immunohistochemistry.In conclusion, the proportion of T helper and cytotoxic cells vs. regulatory T cells in different locations of the tumor microenvironment have opposite prognostic impacts in resected NSCLC.
eng
Immune biomarker
NSCLC
Tumor compartment
Analysis of the immune microenvironment in resected non-small cell lung cancer: the prognostic value of different T lymphocyte markers
journal article
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URL
https://repisalud.isciii.es/bitstreams/5d1220cb-e2d7-4b1e-80b6-00c61e43f102/download
File
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Analysisoftheimmunemicroenvironment_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/2d8542ed-85b8-406d-b62d-646e03f30c1c/download
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Analysisoftheimmunemicroenvironment_2016.pdf.txt