2026-06-14T03:32:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/104552024-09-27T22:50:53Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Sánchez-Jiménez, Carmen
author
Toledano, Víctor
author
Lopez-Galindez, Luis Cecilio
author
Olivares, Isabel
author
Reis Vieira, Catarina
author
Gutiirrez-Rivas, Monica
author
López, C
funder
Ministerio de Ciencia e Innovación (España)
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
funder
Instituto de Salud Carlos III
2020-06-16T08:48:48Z
2020-06-16T08:48:48Z
2013-05
J Gen Virol . 2013 May;94(Pt 5):944-954
10.1099/vir.0.046573-0
1465-2099
The Journal of general virology
23288426
http://hdl.handle.net/20.500.12105/10455
Human immunodeficiency virus type 1 (HIV-1) persistently infected cell lines are characterized by the continuous viral production without cytopathic effect. However, it is not completely clear if this production is contributed only by viral transcription or also by new cycles of viral replication. We studied an HIV-1 persistently infected cell line, designated H61-D, providing evidence of new replication cycles as sustained by: (i) a decrease in viral production, measured by p24 protein, after treatment of the culture with 3'-azydo-3'-deoxythymydine; (ii) detection of new integration events in the course of cell culture, and (iii) finding of two-long-terminal repeat circles in the cells. H61-D cells were not infected by cell-free virus, but infection was possible by co-culture with another productive-infected cell line. In conclusion, ongoing viral replication is taking place in H61-D persistent cells and new infections are mediated by a cell-to-cell spread mechanism.
eng
Evidence of ongoing replication in a human immunodeficiency virus type 1 persistently infected cell line.
journal article
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URL
https://repisalud.isciii.es/bitstreams/fc5bc11f-74e0-47fe-b522-c8729ead56df/download
File
MD5
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application/pdf
EvidenceOfOngoingReplication_2013.pdf
URL
https://repisalud.isciii.es/bitstreams/add3457c-89af-4ea5-9350-24911ac57e80/download
File
MD5
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text/plain
EvidenceOfOngoingReplication_2013.pdf.txt