2026-08-29T12:39:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/104162024-09-27T20:20:31Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Rossey, Iebe
author
Gilman, Morgan S A
author
Kabeche, Stephanie C
author
Sedeyn, Koen
author
Wrapp, Daniel
author
Kanekiyo, Masaru
author
Chen, Man
author
Spitaels, Jan
author
Graham, Barney S
author
Schepens, Bert
author
McLellan, Jason S
author
Saelens, Xavier
author
Mas-Lloret, Vicente
author
Melero, Jose Antonio
funder
National Institutes of Health (Estados Unidos)
funder
Ministerio de Economía y Competitividad (España)
funder
NIH - National Cancer Institute (NCI) (Estados Unidos)
funder
NIH - National Institute of General Medical Sciences (NIGMS) (Estados Unidos)
2020-06-16T07:34:00Z
2020-06-16T07:34:00Z
2017
Nat Commun . 2017 Feb 13;8:14158.
10.1038/ncomms14158
2041-1723
Nature communications
28194013
http://hdl.handle.net/20.500.12105/10416
Human respiratory syncytial virus (RSV) is the main cause of lower respiratory tract infections in young children. The RSV fusion protein (F) is highly conserved and is the only viral membrane protein that is essential for infection. The prefusion conformation of RSV F is considered the most relevant target for antiviral strategies because it is the fusion-competent form of the protein and the primary target of neutralizing activity present in human serum. Here, we describe two llama-derived single-domain antibodies (VHHs) that have potent RSV-neutralizing activity and bind selectively to prefusion RSV F with picomolar affinity. Crystal structures of these VHHs in complex with prefusion F show that they recognize a conserved cavity formed by two F protomers. In addition, the VHHs prevent RSV replication and lung infiltration of inflammatory monocytes and T cells in RSV-challenged mice. These prefusion F-specific VHHs represent promising antiviral agents against RSV.
eng
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state.
journal article
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URL
https://repisalud.isciii.es/bitstreams/e2d34ed2-e75b-482a-b6a3-deeee5abcfae/download
File
MD5
38624afdac1cfecb05519b9e09fd0208
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application/pdf
PotentSingleDomainAntibodies_2017.pdf
URL
https://repisalud.isciii.es/bitstreams/300cb88e-72ba-4f72-9c88-256d8d31f680/download
File
MD5
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text/plain
PotentSingleDomainAntibodies_2017.pdf.txt