2026-06-14T00:39:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/103652024-09-27T20:31:16Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Laguna, Teresa
author
Notario, Laura
author
Pippa, Raffaella
author
Gomez Fontela, Miguel
author
Vázquez, Berta N
author
Maicas, Miren
author
Aguilera-Montilla, Noemí
author
Corbí, Ángel L
author
Odero, María D
author
Lauzurica, Pilar
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Educación y Ciencia (España)
2020-06-12T08:01:49Z
2020-06-12T08:01:49Z
2015-08
Mol Immunol . 2015 Aug;66(2):171-9.
10.1016/j.molimm.2015.02.031
1872-9142
Molecular immunology
25801305
http://hdl.handle.net/20.500.12105/10365
The CD69 type II C-type lectin is one of the earliest indicators of leukocyte activation acting in lymphocyte migration and cytokine secretion. CD69 expression in hematopoietic lineage undergoes rapid changes depending on the cell-lineage, the activation state or the localization of the cell where it is expressed, suggesting a complex and tightly controlled regulation. Here we provide new insights on the transcriptional regulation of CD69 gene in mammal species. Through in silico studies, we analyzed several regulatory features of the 4 upstream conserved non-coding sequences (CNS 1-4) previously described, confirming a major function of CNS2 in the transcriptional regulation of CD69. In addition, multiple transcription binding sites are identified in the CNS2 region by DNA cross-species conservation analysis. By functional approaches we defined a core region of 226bp located within CNS2 as the main enhancer element of CD69 transcription in the hematopoietic cells analyzed. By chromatin immunoprecipitation, binding of RUNX1 to the core-CNS2 was shown in a T cell line. In addition, we found an activating but not essential role of RUNX1 in CD69 gene transcription by site-directed mutagenesis and RNA silencing, probably through the interaction with this potent enhancer specifically in the hematopoietic lineage. In summary, in this study we contribute with new evidences to the landscape of the transcriptional regulation of the CD69 gene.
eng
New insights on the transcriptional regulation of CD69 gene through a potent enhancer located in the conserved non-coding sequence 2.
journal article
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URL
https://repisalud.isciii.es/bitstreams/abbb6e56-e984-4918-bfab-e354998270db/download
File
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application/pdf
NewInsightsOnThe_2015.pdf
URL
https://repisalud.isciii.es/bitstreams/0bf26923-0313-4af7-9b28-d35966b0bdf8/download
File
MD5
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text/plain
NewInsightsOnThe_2015.pdf.txt