2026-06-14T03:53:17Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/103652024-09-27T20:31:16Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Laguna, Teresa author Notario, Laura author Pippa, Raffaella author Gomez Fontela, Miguel author Vázquez, Berta N author Maicas, Miren author Aguilera-Montilla, Noemí author Corbí, Ángel L author Odero, María D author Lauzurica, Pilar author 2015-08 The CD69 type II C-type lectin is one of the earliest indicators of leukocyte activation acting in lymphocyte migration and cytokine secretion. CD69 expression in hematopoietic lineage undergoes rapid changes depending on the cell-lineage, the activation state or the localization of the cell where it is expressed, suggesting a complex and tightly controlled regulation. Here we provide new insights on the transcriptional regulation of CD69 gene in mammal species. Through in silico studies, we analyzed several regulatory features of the 4 upstream conserved non-coding sequences (CNS 1-4) previously described, confirming a major function of CNS2 in the transcriptional regulation of CD69. In addition, multiple transcription binding sites are identified in the CNS2 region by DNA cross-species conservation analysis. By functional approaches we defined a core region of 226bp located within CNS2 as the main enhancer element of CD69 transcription in the hematopoietic cells analyzed. By chromatin immunoprecipitation, binding of RUNX1 to the core-CNS2 was shown in a T cell line. In addition, we found an activating but not essential role of RUNX1 in CD69 gene transcription by site-directed mutagenesis and RNA silencing, probably through the interaction with this potent enhancer specifically in the hematopoietic lineage. In summary, in this study we contribute with new evidences to the landscape of the transcriptional regulation of the CD69 gene. Mol Immunol . 2015 Aug;66(2):171-9. 10.1016/j.molimm.2015.02.031 1872-9142 Molecular immunology 25801305 http://hdl.handle.net/20.500.12105/10365 New insights on the transcriptional regulation of CD69 gene through a potent enhancer located in the conserved non-coding sequence 2.