2026-05-21T23:47:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/103572024-09-27T08:25:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Izidoro, Mario Augusto
author
Cecconi, Alberto
author
Panadero, María Isabel
author
Mateo, Jesus
author
Godzien, Joanna
author
Vilchez, Jean Paul
author
López-Gonzálvez, Ángeles
author
Ruiz-Cabello, Jesus
author
Ibáñez, Borja
author
Barbas, Coral
author
Rupérez, Francisco J
funder
Airbus Defense and Space
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Sociedad Española de Cardiología
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación BBVA
funder
Fundación ProCNIC
2020-06-11T13:35:17Z
2020-06-11T13:35:17Z
2020-04-27
Sci Rep. 2020; 10(1):7072
10.1038/s41598-020-63306-y
2045-2322
Scientific reports
32341369
http://hdl.handle.net/20.500.12105/10357
Balloon catheter endothelial denudation in New Zealand white rabbits fed high cholesterol diet is a validated atherosclerosis model. Well-characterized in terms of atherosclerosis induction and progression, the metabolic changes associated with the atherosclerosis progression remain indeterminate. Non-targeted metabolomics permits to develop such elucidation and allows to evaluate the metabolic consequences of colchicine treatment, an anti-inflammatory drug that could revert these changes. 16 rabbits underwent 18 weeks of atherosclerosis induction by diet and aortic denudation. Thereafter animals were randomly assigned to colchicine treatment or placebo for 18 weeks while on diet. Plasma samples were obtained before randomization and at 36 weeks. Multiplatform (GC/MS, CE/MS, RP-HPLC/MS) metabolomics was applied. Plasma fingerprints were pre-processed, and the resulting matrixes analyzed to unveil differentially expressed features. Different chemical annotation strategies were accomplished for those significant features. We found metabolites associated with either atherosclerosis progression, or colchicine treatment, or both. Atherosclerosis was profoundly associated with an increase in circulating bile acids. Most of the changes associated with sterol metabolism could not be reverted by colchicine treatment. However, the variations in lysine, tryptophan and cysteine metabolism among others, have shown new potential mechanisms of action of the drug, also related to atherosclerosis progression, but not previously described.
eng
Plasma Metabolic Signature of Atherosclerosis Progression and Colchicine Treatment in Rabbits.
journal article
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PlasmaMetabolicSignatureAtherosclerosis_2020.pdf
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PlasmaMetabolicSignatureAtherosclerosis_2020.pdf.txt