2026-08-29T12:56:22Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/103572024-09-27T08:25:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Izidoro, Mario Augusto author Cecconi, Alberto author Panadero, María Isabel author Mateo, Jesus author Godzien, Joanna author Vilchez, Jean Paul author López-Gonzálvez, Ángeles author Ruiz-Cabello, Jesus author Ibáñez, Borja author Barbas, Coral author Rupérez, Francisco J author 2020-04-27 Balloon catheter endothelial denudation in New Zealand white rabbits fed high cholesterol diet is a validated atherosclerosis model. Well-characterized in terms of atherosclerosis induction and progression, the metabolic changes associated with the atherosclerosis progression remain indeterminate. Non-targeted metabolomics permits to develop such elucidation and allows to evaluate the metabolic consequences of colchicine treatment, an anti-inflammatory drug that could revert these changes. 16 rabbits underwent 18 weeks of atherosclerosis induction by diet and aortic denudation. Thereafter animals were randomly assigned to colchicine treatment or placebo for 18 weeks while on diet. Plasma samples were obtained before randomization and at 36 weeks. Multiplatform (GC/MS, CE/MS, RP-HPLC/MS) metabolomics was applied. Plasma fingerprints were pre-processed, and the resulting matrixes analyzed to unveil differentially expressed features. Different chemical annotation strategies were accomplished for those significant features. We found metabolites associated with either atherosclerosis progression, or colchicine treatment, or both. Atherosclerosis was profoundly associated with an increase in circulating bile acids. Most of the changes associated with sterol metabolism could not be reverted by colchicine treatment. However, the variations in lysine, tryptophan and cysteine metabolism among others, have shown new potential mechanisms of action of the drug, also related to atherosclerosis progression, but not previously described. Sci Rep. 2020; 10(1):7072 10.1038/s41598-020-63306-y 2045-2322 Scientific reports 32341369 http://hdl.handle.net/20.500.12105/10357 Plasma Metabolic Signature of Atherosclerosis Progression and Colchicine Treatment in Rabbits.