2026-06-30T02:01:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/103412024-10-31T11:42:28Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Ruiz, Susana
author
Domenech, Mirian
author
Antequera, María Luisa
author
García, Pedro
author
García, Ernesto
author
Corsini, Bruno
author
Aguinagalde, Leire
author
Fenoll, Asuncion
author
Yuste, Jose Enrique
funder
Ministerio de Economía y Competitividad (España)
funder
Centro de Investigación Biomedica en Red - CIBER
funder
National Council for Scientific and Technological Development (Brasil)
2020-06-11T09:21:39Z
2020-06-11T09:21:39Z
2016
Vaccine. 2016 Dec 7;34(50):6148-6157.
10.1016/j.vaccine.2016.11.001
1873-2518
Vaccine
27840016
http://hdl.handle.net/20.500.12105/10341
The cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice with LytB in the presence of alhydrogel as an adjuvant. Enzyme-linked immunosorbent assays measuring different subclasses of immunoglobulin G, demonstrated that the antibody responses to LytB were predominantly IgG1 and IgG2b, followed by IgG3 and IgG2a subclasses. Complement-mediated immunity against two different pneumococcal serotypes was investigated using sera from immunized mice. Immunization with LytB increased the recognition of S. pneumoniae by complement components C1q and C3b demonstrating that anti-LytB antibodies trigger activation of the classical pathway. Phagocytosis assays showed that serum containing antibodies to LytB stimulates neutrophil-mediated phagocytosis against S. pneumoniae. Animal models of infection including invasive pneumonia and sepsis were performed with two different clinical isolates. Vaccination with LytB increased bacterial clearance and induced protection demonstrating that LytB might be a good candidate to be considered in a future protein-based vaccine against S. pneumoniae.
eng
Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
journal article
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URL
https://repisalud.isciii.es/bitstreams/da6107e8-9059-4902-b268-33ced362dafb/download
File
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ImmunizationWithLytBProtein_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/7d8468e4-e4a0-4b32-8df5-48f2098d204e/download
File
MD5
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ImmunizationWithLytBProtein_2016.pdf.txt