2026-04-27T01:59:19Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/103402025-05-08T14:52:04Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Ayats-Ardite, Josefina
author
Castro, Carmen
author
García-Rodríguez, Julio
author
Goterris-Bonet, Lidia
author
Ibáñez-Martínez, Elisa
author
Linares-Sicilia, María José
author
Martín-Gómez, María Teresa
author
Martín-Mazuelos, Estrella
author
Pelaez, Teresa
author
Pemán, Javier
author
Rezusta, Antonio
author
Rojo, Susana
author
Tejero, Rocío
author
Anza, Diego Vicente
author
Viñuelas, Jesús
author
Zapico, Maria Soledad
author
Alastruey-Izquierdo, Ana
author
Alcazar-Fuoli, Laura
author
Rivero-Menendez, Olga
author
Cuenca-Estrella, Manuel
funder
Instituto de Salud Carlos III
2020-06-11T09:11:57Z
2020-06-11T09:11:57Z
2018
Antimicrob Agents Chemother . 2018 Aug 27;62(9):e00358-18.
http://hdl.handle.net/20.500.12105/10340
29941643
10.1128/AAC.00358-18
1098-6596
Antimicrobial agents and chemotherapy
Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergillus fumigatus was not detected. We used itraconazole (ITZ)-supplemented medium to select resistant strains. A total of 500 plates supplemented with 2 mg/liter of ITZ were sent to 10 Spanish tertiary hospitals, and molecular identification and antifungal susceptibility testing were performed. In addition, the cyp51A gene in those A. fumigatus strains showing azole resistance was sequenced. A total of 493 isolates were included in the study. Sixteen strains were isolated from patients with an infection classified as proven, 104 were isolated from patients with an infection classified as probable, and 373 were isolated from patients with an infection classified as colonization. Aspergillus was the most frequent genus isolated, at 80.3%, followed by Scedosporium-Lomentospora (7.9%), Penicillium-Talaromyces (4.5%), Fusarium (2.6%), and the order Mucorales (1%). Antifungal resistance was detected in Scedosporium-Lomentospora species, Fusarium, Talaromyces, and Mucorales Three strains of A. fumigatus sensu stricto were resistant to azoles; two of them harbored the TR34+L98H mechanism of resistance, and the other one had no mutations in cyp51A The level of azole resistance in A. fumigatus remains low, but cryptic species represent over 10% of the isolates and have a broader but overall higher range of antifungal resistance.
eng
Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).
research article
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MolecularIdentificationAndSusceptibility_2018.pdf
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https://repisalud.isciii.es/bitstreams/1a7df708-f1a3-4003-95b7-81fc44a61821/download
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cd5d057dde31b10b9a647438a7bd8bdb
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text/plain
MolecularIdentificationAndSusceptibility_2018.pdf.txt