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oai:repisalud.isciii.es:20.500.12105/103402025-05-08T14:52:04Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Ayats-Ardite, Josefina author Castro, Carmen author García-Rodríguez, Julio author Goterris-Bonet, Lidia author Ibáñez-Martínez, Elisa author Linares-Sicilia, María José author Martín-Gómez, María Teresa author Martín-Mazuelos, Estrella author Pelaez, Teresa author Pemán, Javier author Rezusta, Antonio author Rojo, Susana author Tejero, Rocío author Anza, Diego Vicente author Viñuelas, Jesús author Zapico, Maria Soledad author Alastruey-Izquierdo, Ana author Alcazar-Fuoli, Laura author Rivero-Menendez, Olga author Cuenca-Estrella, Manuel author 2018 Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds of between 10 and 12.7%, but secondary resistance in Aspergillus fumigatus was not detected. We used itraconazole (ITZ)-supplemented medium to select resistant strains. A total of 500 plates supplemented with 2 mg/liter of ITZ were sent to 10 Spanish tertiary hospitals, and molecular identification and antifungal susceptibility testing were performed. In addition, the cyp51A gene in those A. fumigatus strains showing azole resistance was sequenced. A total of 493 isolates were included in the study. Sixteen strains were isolated from patients with an infection classified as proven, 104 were isolated from patients with an infection classified as probable, and 373 were isolated from patients with an infection classified as colonization. Aspergillus was the most frequent genus isolated, at 80.3%, followed by Scedosporium-Lomentospora (7.9%), Penicillium-Talaromyces (4.5%), Fusarium (2.6%), and the order Mucorales (1%). Antifungal resistance was detected in Scedosporium-Lomentospora species, Fusarium, Talaromyces, and Mucorales Three strains of A. fumigatus sensu stricto were resistant to azoles; two of them harbored the TR34+L98H mechanism of resistance, and the other one had no mutations in cyp51A The level of azole resistance in A. fumigatus remains low, but cryptic species represent over 10% of the isolates and have a broader but overall higher range of antifungal resistance. Antimicrob Agents Chemother . 2018 Aug 27;62(9):e00358-18. http://hdl.handle.net/20.500.12105/10340 29941643 10.1128/AAC.00358-18 1098-6596 Antimicrobial agents and chemotherapy Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study).