2026-04-29T22:45:40Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/102922025-04-24T18:42:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Luz, Sergio LB author Merino, Francisco J author López-Vélez, Rogelio author Almeida, Tatiana A P author Abrahim, Cláudia M M author Ta Tang, Thuy-Huong author Fuentes Corripio, Isabel author Lanza-Suarez, Marta author Rubio Muñoz, Jose Miguel author 2016 Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. Mansonella ozzardi and Mansonella perstans have been reported in Latin America, including the Amazon region. A morphological and molecular microfilariae study was performed in Pauini (Brazil). Blood samples were collected from 40 individuals, and were analyzed by Giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm protein gene. By microscopy, 14 of 40 were positive: 11 as M. ozzardi and three as M. perstans-like infections. Both molecular methods detected 19 positive cases as M. ozzardi, including those 14 individuals detected by microscopy, without detectable genetic differences among any of the 19 positive samples. Molecular techniques showed an improvement of mansonellosis diagnosis and may become an effective tool to evaluate the present status of M. ozzardi and M. perstans in Latin America. Am J Trop Med Hyg . 2016 Sep 7;95(3):629-32. 10.4269/ajtmh.15-0654 1476-1645 The American journal of tropical medicine and hygiene 27402517 http://hdl.handle.net/20.500.12105/10292 Atypical Mansonella ozzardi Microfilariae from an Endemic Area of Brazilian Amazonia.