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oai:repisalud.isciii.es:20.500.12105/102462024-11-28T16:01:01Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_2173com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_19597col_20.500.12105_16938

00925njm 22002777a 4500 dc Aragoneses-Fenoll, Laura author Montes-Casado, Maria author Ojeda, Gloria author Acosta, Y Y author Herranz, J author Martinez, Sonia author Blanco-Aparicio, Carmen author Criado, G author Pastor Fernandez, Joaquin author Dianzani, U author Portoles, Pilar author Rojo, J M author 2016-04-15 Class IA phosphoinositide 3-kinases (PI3Ks) are essential to function of normal and tumor cells, and to modulate immune responses. T lymphocytes express high levels of p110α and p110δ class IA PI3K. Whereas the functioning of PI3K p110δ in immune and autoimmune reactions is well established, the role of p110α is less well understood. Here, a novel dual p110α/δ inhibitor (ETP-46321) and highly specific p110α (A66) or p110δ (IC87114) inhibitors have been compared concerning T cell activation in vitro, as well as the effect on responses to protein antigen and collagen-induced arthritis in vivo. In vitro activation of naive CD4(+) T lymphocytes by anti-CD3 and anti-CD28 was inhibited more effectively by the p110δ inhibitor than by the p110α inhibitor as measured by cytokine secretion (IL-2, IL-10, and IFN-γ), T-bet expression and NFAT activation. In activated CD4(+) T cells re-stimulated through CD3 and ICOS, IC87114 inhibited Akt and Erk activation, and the secretion of IL-2, IL-4, IL-17A, and IFN-γ better than A66. The p110α/δ inhibitor ETP-46321, or p110α plus p110δ inhibitors also inhibited IL-21 secretion by differentiated CD4(+) T follicular (Tfh) or IL-17-producing (Th17) helper cells. In vivo, therapeutic administration of ETP-46321 significantly inhibited responses to protein antigen as well as collagen-induced arthritis, as measured by antigen-specific antibody responses, secretion of IL-10, IL-17A or IFN-γ, or clinical symptoms. Hence, p110α as well as p110δ Class IA PI3Ks are important to immune regulation; inhibition of both subunits may be an effective therapeutic approach in inflammatory autoimmune diseases like rheumatoid arthritis. Biochem Pharmacol. 2016 Apr 15;106:56-69. 10.1016/j.bcp.2016.02.005 1873-2968 0006-2952 Biochemical pharmacology 26883061 http://hdl.handle.net/20.500.12105/10246 A-66 CD28 ETP-46321 IC-87114 ICOS PI3K inhibitors Phosphatidyl inositol-3 kinase Rheumatoid arthritis T lymphocytes ETP-46321, a dual p110α/δ class IA phosphoinositide 3-kinase inhibitor modulates T lymphocyte activation and collagen-induced arthritis