2026-06-14T03:39:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/101582025-06-09T07:40:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19617
Repisalud
author
Quintana, Elena
author
Fernandez-Ramos, Andres
author
Velasco, Patricia
author
Andres, Belen de
author
Liste-Noya, Isabel
author
Sancho, David
author
Gaspar, Maria Luisa
author
Cano, Eva
funder
Instituto de Salud Carlos III
funder
Red Temática de Investigación Cooperativa en Cáncer (RTICC) (España)
2020-05-18T10:22:38Z
2020-05-18T10:22:38Z
2015-12
Glia. 2015 Dec;63(12):2231-48.
10.1002/glia.22889
1098-1136
0894-1491
Glia
26184558
http://hdl.handle.net/20.500.12105/10158
The role and different origin of brain myeloid cells in the brain is central to understanding how the central nervous system (CNS) responds to injury. C-type lectin receptor family 9, member A (DNGR-1/CLEC9A) is a marker of specific DC subsets that share functional similarities, such as CD8α(+) DCs in lymphoid tissues and CD103(+) CD11b(low) DCs in peripheral tissues. Here, we analyzed the presence of DNGR-1 in DCs present in the mouse brain (bDCs). Dngr-1/Clec9a mRNA is expressed mainly in the meningeal membranes and choroid plexus (m/Ch), and its expression is enhanced by fms-like tyrosine kinase 3 ligand (Flt3L), a cytokine involved in DC homeostasis. Using Clec9a(egfp/egfp) mice, we show that Flt3L induces accumulation of DNGR-1-EGFP(+) cells in the brain m/Ch. Most of these cells also express major histocompatibility complex class II (MHCII) molecules. We also observed an increase in specific markers of cDC CD8α+ cells such as Batf-3 and Irf-8, but not of costimulatory molecules such as Cd80 and Cd86, indicating an immature phenotype for these bDCs in the noninjured brain. The presence of DNGR-1 in the brain provides a potential marker for the study of this specific brain cell subset. Knowledge and targeting of brain antigen presenting cells (APCs) has implications for the fight against brain diseases such as neuroinflammation-based neurodegenerative diseases, microbe-induced encephalitis, and brain tumors such as gliomas.
eng
Batf3
C-type lectin receptors
CD11c
Flt3L
Myeloid cells
Neuroinflammation
DNGR-1(+) dendritic cells are located in meningeal membrane and choroid plexus of the noninjured brain
preprint
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DNGR-1(+)DendriticCells_2015.pdf
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DNGR_1+DendriticCellsAre_2015.pdf.txt
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DNGR-1(+)DendriticCells_2015.pdf.txt