2026-06-14T03:46:48Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/101502024-09-27T23:42:30Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Dwibedi, Chinmay author Birdsell, Dawn author Lärkeryd, Adrian author Myrtennäs, Kerstin author Öhrman, Caroline author Nilsson, Elin author Karlsson, Edvin author Hochhalter, Christian author Rivera, Andrew author Maltinsky, Sara author Bayer, Brittany author Keim, Paul author Scholz, Holger C author Tomaso, Herbert author Wittwer, Matthias author Beuret, Christian author Schuerch, Nadia author Pilo, Paola author Hernández Pérez, Marta author Rodríguez-Lázaro, David author Escudero, Raquel author Anda, Pedro author Forsman, Mats author Wagner, David M author Larsson, Pär author Johansson, Anders author 2016 For many infections transmitting to humans from reservoirs in nature, disease dispersal patterns over space and time are largely unknown. Here, a reversed genomics approach helped us understand disease dispersal and yielded insight into evolution and biological properties of Francisella tularensis, the bacterium causing tularemia. We whole-genome sequenced 67 strains and characterized by single-nucleotide polymorphism assays 138 strains, collected from individuals infected 1947-2012 across Western Europe. We used the data for phylogenetic, population genetic and geographical network analyses. All strains (n=205) belonged to a monophyletic population of recent ancestry not found outside Western Europe. Most strains (n=195) throughout the study area were assigned to a star-like phylogenetic pattern indicating that colonization of Western Europe occurred via clonal expansion. In the East of the study area, strains were more diverse, consistent with a founder population spreading from east to west. The relationship of genetic and geographic distance within the F. tularensis population was complex and indicated multiple long-distance dispersal events. Mutation rate estimates based on year of isolation indicated null rates; in outbreak hotspots only, there was a rate of 0.4 mutations/genome/year. Patterns of nucleotide substitution showed marked AT mutational bias suggestive of genetic drift. These results demonstrate that tularemia has moved from east to west in Europe and that F. tularensis has a biology characterized by long-range geographical dispersal events and mostly slow, but variable, replication rates. The results indicate that mutation-driven evolution, a resting survival phase, genetic drift and long-distance geographical dispersal events have interacted to generate genetic diversity within this species. Microb Genom. 2016 Dec 12;2(12):e000100. 10.1099/mgen.0.000100 2057-5858 Microbial genomics 28348839 http://hdl.handle.net/20.500.12105/10150 Francisella tularensis Disease transmission Epidemiology Genetic variation Human Population genetics Long-range dispersal moved Francisella tularensis into Western Europe from the East