2026-06-14T10:38:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/100062024-11-29T23:37:12Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Shakiba, Nika
author
White, Carl A
author
Lipsitz, Yonatan Y
author
Yachie-Kinoshita, Ayako
author
Tonge, Peter D
author
Hussein, Samer M I
author
Puri, Mira C
author
Elbaz, Judith
author
Morrissey-Scoot, James
author
Li, Mira
author
Munoz, Javier
author
Benevento, Marco
author
Rogers, Ian M
author
Hanna, Jacob H
author
Heck, Albert J R
author
Wollscheid, Bernd
author
Nagy, Andras
author
Zandstra, Peter W
funder
Ontario Government
funder
Canadian Institutes of Health Research
funder
NSERC Vanier Canada Graduate Scholarship
2020-05-08T17:20:59Z
2020-05-08T17:20:59Z
2015-06-16
Nat Commun. 2015;6:7329.
10.1038/ncomms8329
2041-1723
2041-1723
Nature communications
26076835
http://hdl.handle.net/20.500.12105/10006
Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.
eng
CD24 tracks divergent pluripotent states in mouse and human cells
journal article
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