2026-06-14T10:38:51Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/100062024-11-29T23:37:12Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Shakiba, Nika author White, Carl A author Lipsitz, Yonatan Y author Yachie-Kinoshita, Ayako author Tonge, Peter D author Hussein, Samer M I author Puri, Mira C author Elbaz, Judith author Morrissey-Scoot, James author Li, Mira author Munoz, Javier author Benevento, Marco author Rogers, Ian M author Hanna, Jacob H author Heck, Albert J R author Wollscheid, Bernd author Nagy, Andras author Zandstra, Peter W author 2015-06-16 Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture. Nat Commun. 2015;6:7329. 10.1038/ncomms8329 2041-1723 2041-1723 Nature communications 26076835 http://hdl.handle.net/20.500.12105/10006 CD24 tracks divergent pluripotent states in mouse and human cells