TY - JOUR AU - Bortel, Patricia AU - Piga, Ilaria AU - Koenig, Claire AU - Gerner, Christopher AU - Martinez-Val, Ana AU - Olsen, Jesper V PY - 2024 DO - 10.1016/j.mcpro.2024.100754 UR - http://hdl.handle.net/20.500.12105/20077 AB - Improving coverage, robustness, and sensitivity is crucial for routine phosphoproteomics analysis by single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) from minimal peptide inputs. Here, we systematically optimized key experimental... LA - eng PB - Elsevier KW - Phosphopeptides KW - Proteomics KW - Tandem Mass Spectrometry KW - Phosphoproteins KW - Humans KW - Chromatography, Liquid KW - HeLa Cells KW - Proteome KW - Phosphorylation KW - Automation TI - Systematic Optimization of Automated Phosphopeptide Enrichment for High-Sensitivity Phosphoproteomics. TY - journal article ER -