TY  - GEN
AU  - Toraño, Alfredo
AU  - Moreno-Iruela, Inmaculada
AU  - Infantes-Lorenzo, Jose Antonio
AU  - Dominguez-Rodriguez, Mercedes
PY  - 2024
DO  - 10.1016/j.jim.2024.113756
SN  - 0022-1759
UR  - https://hdl.handle.net/20.500.12105/26429
AB  - We present a time-course saturation ELISA for measuring the equilibrium constant of the monoclonal antibody (mAb) SIM 28 against horse radish peroxidase (HRP). The curves of HRP binding to a series of fixed mAb dilutions were plotted to completion,...
LA  - eng
PB  - Elsevier
KW  - Monoclonal antibody affinity determination
KW  - Noncompetitive inhibition kinetics
KW  - Solid-phase time-course saturation ELISA
KW  - Animals
KW  - Antibodies, Monoclonal
KW  - Antibody Affinity
KW  - Enzyme-Linked Immunosorbent Assay
KW  - Horseradish Peroxidase
KW  - Kinetics
KW  - Ligands
KW  - Time Factors
TI  - Description of a non-competitive ELISA based on time course analysis of ligand binding at saturation, and a direct method for calculating the affinity of monoclonal antibodies
TY  - research article
ER  -