TY - JOUR AU - Orbegozo-Medina, Ricardo A AU - Martínez-Sernández, Victoria AU - Perteguer-Prieto, Maria Jesus AU - Hernandez-Gonzalez, Ana AU - Mezo, Mercedes AU - González-Warleta, Marta AU - Romarís, Fernanda AU - Paniagua, Esperanza AU - Garate, Teresa AU - Ubeira, Florencio M PY - 2019 DO - 10.1371/journal.pone.0211035 SN - 1932-6203 UR - http://hdl.handle.net/20.500.12105/7443 AB - Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but... LA - eng PB - Public Library of Science (PLOS) TI - In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA TY - journal article ER -