TY - JOUR AU - Navarro-Lerida, Inmaculada AU - Alvarez-Barrientos, Alberto AU - Gavilanes, Francisco AU - Rodriguez-Crespo, Ignacio PY - 2002 DO - 10.1242/jcs.209973 SN - 0021-9533 UR - http://hdl.handle.net/20.500.12105/11915 AB - Using recursive PCR, we created an artificial protein sequence that consists of a consensus myristoylation motif (MGCTLS) followed by the triplet AGS repeated nine times and fused to the GFP reporter. This linker-GFP sequence was utilized as a base to... LA - eng PB - The Company of Biologists KW - Recombinant Fusion Proteins KW - Amino Acid Sequence KW - Animals KW - COS Cells KW - Caveolin 1 KW - Caveolins KW - Cell Compartmentation KW - Cell Membrane KW - Cholesterol KW - Cytoplasm KW - Eukaryotic Cells KW - Golgi Apparatus KW - Green Fluorescent Proteins KW - Luminescent Proteins KW - Membrane Microdomains KW - Microscopy, Confocal KW - Myristic Acid KW - Octoxynol KW - Palmitates KW - Palmitic Acid KW - Protein Transport KW - Proteins KW - Solubility KW - Sphingomyelins TI - Distance-dependent cellular palmitoylation of de-novo-designed sequences and their translocation to plasma membrane subdomains. TY - journal article ER -