TY - JOUR AU - Martin-Galiano, Antonio Javier AU - de la Campa, Adela G PY - 2003 DO - 10.1128/aac.47.4.1257-1261.2003 SN - 0066-4804 UR - http://hdl.handle.net/20.500.12105/8236 AB - We designed a method by which to generate antibiotic-resistant strains of Streptococcus pneumoniae at frequencies 4 orders of magnitude greater than the spontaneous mutation rate. The method is based on the natural ability of this organism to be... LA - eng PB - American Society for Microbiology (ASM) KW - Amino Acid Sequence KW - Ciprofloxacin KW - Molecular Sequence Data KW - Polymerase Chain Reaction KW - Rifampin KW - Streptococcus pneumoniae KW - Streptomycin KW - Drug Resistance, Bacterial KW - Transformation, Bacterial TI - High-efficiency generation of antibiotic-resistant strains of Streptococcus pneumoniae by PCR and transformation TY - journal article ER -