TY - GEN AU - Pérez-García, Felipe AU - Virseda-Berdices, Ana AU - Pita-Martínez, Carlos AU - Muñoz Monte, Mario AU - Sepulveda-Crespo, Daniel AU - Codina Márquez, Helena AU - Alonso, Roberto AU - Mesones, Lara AU - Rodrigo, Sandra AU - Macías, Juan AU - Real, Luis Miguel AU - Cuadros-González, Juan AU - Martinez, Isidoro AU - Resino, Salvador PY - 2026 DO - 10.1128/jcm.01517-25 UR - https://hdl.handle.net/20.500.12105/27567 AB - Quantitative RT-PCR (qRT-PCR) is essential for monitoring hepatitis delta virus (HDV) RNA, yet assays lack standardization. We aimed to evaluate the performance of three qRT-PCR assays and to assess the impact of a pre-analytical thermal shock... LA - eng PB - American Society for Microbiology (ASM) KW - Hepatitis D virus KW - Inter-assay variability KW - qRT-PCR KW - Thermal shock KW - Viral load KW - Hepatitis D KW - Hepatitis Delta Virus KW - Humans TI - Challenges in accurate HDV RNA quantification: inter-assay variability and the impact of thermal shock. TY - research article ER -