2024-03-28T11:52:43Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/99792022-11-07T14:54:37Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2146
Repisalud
author
Marinaro, Federica
author
Macías-García, Beatriz
author
Sánchez-Margallo, Francisco Miguel
author
Blázquez, Rebeca
author
Alvarez, Veronica
author
Matilla, Elvira
author
Hernández, Nuria
author
Gomez-Serrano, Maria
author
Jorge, Inmaculada
author
Vazquez, Jesus
author
González-Fernández, Lauro
author
Pericuesta, Eva
author
Gutiérrez-Adán, Alfonso
author
Casado, Javier G
funder
Instituto de Salud Carlos III
2020-05-08T08:56:07Z
2020-05-08T08:56:07Z
2019-05
Biol Reprod. 2019; 100(5):1180-92
0006-3363
http://hdl.handle.net/20.500.12105/9979
30596891
10.1093/biolre/ioy263
1529-7268
Biology of reproduction
Advanced age is a risk factor undermining women's fertility. Hence, the optimization of assisted reproduction techniques is an interdisciplinary challenge that requires the improvement of in vitro culture systems. Here, we hypothesize that supplementation of embryo culture medium with extracellular vesicles from endometrial-derived mesenchymal stem cells (EV-endMSCs) may have a positive impact on the embryo competence of aged oocytes. In this work, 24 weeks old B6D2 female mice were used as egg donors and in vitro fertilization assays were performed using males from the same strain (8-12 weeks); the presumptive zygotes were incubated in the presence of 0, 10, 20, 40, or 80 μg/ml of EV-endMSCs. The results from the proteomic analysis of EV-endMSCs and the classification by Reactome pathways allowed us to identify proteins closely related with the fertilization process. Moreover, in our aged murine model, the supplementation of the embryo culture medium with EV-endMSCs improved the developmental competence of the embryos as well as the total blastomere count. Finally, gene expression analysis of murine blastocysts showed significant changes on core genes related to cellular response to oxidative stress, metabolism, placentation, and trophectoderm/inner cell mass formation. In summary, we demonstrate that EV-endMSCs increase the quality of the embryos, and according to proteomic and genomic analysis, presumably by modulating the expression of antioxidant enzymes and promoting pluripotent activity. Therefore, EV-endMSCs could be a valuable tool in human assisted reproduction improving the developmental competence of aged oocytes and increasing the odds of implantation and subsequent delivery.
eng
Aging
Embryo culture
Gene expression
Oxidative stress
Proteomics
Stem cells
Extracellular vesicles derived from endometrial human mesenchymal stem cells enhance embryo yield and quality in an aged murine model†
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9979/1/ExtracellularVesiclesDerivedEndometrial_2019.pdf
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ExtracellularVesiclesDerivedEndometrial_2019.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/9979/5/ExtracellularVesiclesDerivedEndometrial_2019.pdf.txt
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ExtracellularVesiclesDerivedEndometrial_2019.pdf.txt