2024-03-29T01:04:57Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/99522022-11-03T14:20:23Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
González-Reyes, L
author
Ruiz-Argüello, M B
author
Garcia-Barreno, Blanca
author
Calder, L
author
López, J A
author
Albar, Juan Pablo
author
Skehel, J J
author
Wiley, D C
author
Melero, Jose Antonio
funder
Ministerio de Ciencia y Tecnología (España)
funder
Unión Europea
funder
Medical Research Council (Reino Unido)
funder
Howard Hughes Medical Institute
2020-05-07T12:36:37Z
2020-05-07T12:36:37Z
2001-08-14
Proc Natl Acad Sci USA. 2001 Aug 14;98(17):9859-64.
0027-8424
http://hdl.handle.net/20.500.12105/9952
11493675
10.1073/pnas.151098198
Proceedings of the National Academy of Sciences of the United States of America
Preparations of purified full-length fusion (F) protein of human respiratory syncytial virus (HRSV) expressed in recombinant vaccinia-F infected cells, or of an anchorless mutant (F(TM(-))) lacking the C-terminal 50 amino acids secreted from vaccinia-F(TM(-))-infected cells contain a minor polypeptide that is an intermediate product of proteolytic processing of the F protein precursor F0. N-terminal sequencing of the intermediate demonstrated that it is generated by cleavage at a furin-motif, residues 106-109 of the F sequence. By contrast, the F1 N terminus derives from cleavage at residue 137 of F0 which is also C-terminal to a furin recognition site at residues 131-136. Site-directed mutagenesis indicates that processing of F0 protein involves independent cleavage at both sites. Both cleavages are required for the F protein to be active in membrane fusion as judged by syncytia formation, and they allow changes in F structure from cone- to lollipop-shaped spikes and the formation of rosettes by anchorless F.
eng
Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9952/1/CleavageOfTheHuman_2001.pdf
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CleavageOfTheHuman_2001.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/9952/3/CleavageOfTheHuman_2001.pdf.txt
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CleavageOfTheHuman_2001.pdf.txt