2024-03-28T08:47:41Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/94482023-02-27T18:06:41Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16988col_20.500.12105_16938col_20.500.12105_2061
Repisalud
author
Solana, Jose Carlos
author
Ramírez, Laura
author
Cook, Emma Cl
author
Hernández-García, Elena
author
Sacristán, Silvia
author
Martín, M Elena
author
Manuel González, Víctor
author
Reguera, Rosa María
author
Balaña-Fouce, Rafael
author
Fresno, Manuel
author
Requena, José María
author
Iborra, Salvador
author
Soto, Manuel
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Fundación Ramón Areces
funder
Banco Santander
2020-04-07T07:40:37Z
2020-04-07T07:40:37Z
2020-03-23
Vaccines (Basel). 2020 Mar 23;8(1). pii: E141.
2076-393X
http://hdl.handle.net/20.500.12105/9448
32210040
10.3390/vaccines8010141
Vaccines
Leishmania infantum parasites cause a severe form of visceral leishmaniasis in human and viscerocutaneous leishmaniasis in dogs. Recently, we reported that immunization with an attenuated L. infantum cell line, lacking the hsp70-II gene, protects against the development of murine cutaneous leishmaniasis. In this work, we analyzed the vaccine potential of this cell line towards the long-term protection against murine visceral leishmaniasis. This model shows an organ-dependent evolution of the disease. The infection can resolve in the liver but chronically affect spleen and bone marrow. Twelve weeks after subcutaneous administration of attenuated L. infantum, Bagg Albino (BALB/c) mice were challenged with infective L. infantum parasites expressing the luciferase-encoding gene. Combining in vivo bioimaging techniques with limiting dilution experiments, we report that, in the initial phase of the disease, vaccinated animals presented lower parasite loads than unvaccinated animals. A reduction of the severity of liver damage was also detected. Protection was associated with the induction of rapid parasite-specific IFN-γ production by CD4+ and CD8+ T cells. However, the vaccine was unable to control the chronic phase of the disease, since we did not find differences in the parasite burdens nor in the immune response at that time point.
eng
IFN-γ
T-cells
Attenuated parasites
Mice
Vaccine
Visceral leishmaniasis
Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9448/1/SubcutaneousImmunizationOfLeishmania_2020.pdf
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9448/5/SubcutaneousImmunizationOfLeishmania_2020.pdf.txt
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SubcutaneousImmunizationOfLeishmania_2020.pdf.txt