2024-03-29T00:44:37Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/92782022-10-06T13:12:10Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Perez-Sautu, Unai
author
Wiley, Michael Ross
author
Iglesias-Caballero, Maria
author
Pozo Sanchez, Francisco
author
Prieto, Karla
author
Chitty, Joseph Alex
author
García-García, María Luz
author
Calvo, Cristina
author
Casas Flecha, Inmaculada
author
Palacios, Gustavo
funder
Defense Threat Reduction Agency (Estados Unidos)
funder
Instituto de Salud Carlos III
2020-03-18T11:21:38Z
2020-03-18T11:21:38Z
2019
Emerg Microbes Infect. 2019;8(1):1054-1065.
2222-1751
http://hdl.handle.net/20.500.12105/9278
31335277
10.1080/22221751.2019.1640587
2222-1751
Emerging microbes & infections
Despite the advanced PCR-based assays available, a fraction of the pediatric respiratory infections remain unexplained every epidemic season, and there is a perception that novel viruses might be present in these specimens. We systematically collected samples from a prospective cohort of pediatric patients with respiratory infections, that returned negative results by validated molecular RT-PCR assays, and studied them with a target-independent, high-throughput sequencing-based approach. We also included a matched cohort of children with no symptoms of respiratory infection, as a contrast study population. More than fifty percent of the specimens from the group of patients with unexplained respiratory infections were resolved. However, the higher rate of detection was not due to the presence of novel viruses, but to the identification of well-known viral respiratory pathogens. Our results show that already known viral pathogens are responsible for the majority of cases that remain unexplained after the epidemic season. High-throughput sequencing approaches that use pathogen-specific probes are easier to standardize because they ensure reproducible library enrichment and sequencing. In consequence, these techniques might be desirable from a regulatory standpoint for diagnostic laboratories seeking to benefit from the many advantages of these sequencing technologies.
eng
Pediatric respiratory infection
High-throughput sequencing
Metagenomics
Respiratory viruses
Viral genomics
Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9278/1/TargetIndependentHhighThroughput_2019.pdf
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TargetIndependentHhighThroughput_2019.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/9278/5/TargetIndependentHhighThroughput_2019.pdf.txt
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TargetIndependentHhighThroughput_2019.pdf.txt