2024-03-28T22:12:18Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/92672022-10-25T11:03:42Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Sarker, Subir
author
Terrón-Orellana, Maria Carmen
author
Khandokar, Yogesh
author
Aragão, David
author
Hardy, Joshua M
author
Radjainia, Mazdak
author
Jiménez-Zaragoza, Manuel
author
de Pablo, Pedro J
author
Coulibaly, Fasséli
author
Luque, Daniel
author
Raidal, Shane R
author
Forwood, Jade K
2020-03-18T07:49:30Z
2020-03-18T07:49:30Z
2016
Nat Commun. 2016 Oct 4;7:13014.
2041-1723
http://hdl.handle.net/20.500.12105/9267
27698405
10.1038/ncomms13014
2041-1723
Nature communications
The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding interfaces distinct from the highly positively charged N-terminal ARM domain.
eng
Structural insights into the assembly and regulation of distinct viral capsid complexes
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9267/1/StructuralInsightsIntoThe_2016.pdf
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https://repisalud.isciii.es/bitstream/20.500.12105/9267/3/StructuralInsightsIntoThe_2016.pdf.txt
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StructuralInsightsIntoThe_2016.pdf.txt