2024-03-29T06:16:58Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/91512023-11-02T17:53:22Zcom_20.500.12105_2067com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2068
Repisalud
author
Mora, Azucena
author
García-Peña, Francisco Javier
author
Alonso, María Pilar
author
Pedraza-Diaz, Susana
author
Ortega-Mora, Luis Miguel
author
Garcia-Parraga, Daniel
author
López, Cecilia
author
Viso, Susana
author
Dahbi, Ghizlane
author
Marzoa, Juan
author
Sergeant, Martin J
author
García, Vanesa
author
Blanco, Jorge
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
Xunta de Galicia (España)
2020-02-28T07:56:05Z
2020-02-28T07:56:05Z
2018
Sci Rep. 2018 Mar 16;8(1):4678.
2045-2322
http://hdl.handle.net/20.500.12105/9151
29549276
10.1038/s41598-018-22943-0
2045-2322
Scientific reports
There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E. coli. Noteworthy, 62 of the 158 E. coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43-fimH22-virotype D; one O16:H5-ST131/PST506-fimH41; one O25:H4-ST6252/PST9-fimH22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates (H22 and H41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E. coli clonal groups, including ST131, in Antarctic pinnipeds.
eng
Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9151/1/ImpactOfHumanAssociated_2018.pdf
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9151/3/ImpactOfHumanAssociated_2018.pdf.txt
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ImpactOfHumanAssociated_2018.pdf.txt