2024-03-28T20:55:12Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/90882023-06-30T11:15:30Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Trevijano-Contador, Nuria
author
Herrero-Fernandez, Ines
author
Garcia-Barbazan, Irene
author
Scorzoni, Liliana
author
Rueda Hernandez, Maria Cristina
author
Rossi, Suelen Andreia
author
Garcia-Rodas, Rocio
author
Zaragoza, Oscar
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Complutense University of Madrid (España)
2020-02-13T11:05:49Z
2020-02-13T11:05:49Z
2015
Virulence. 2015;6(1):66-74.
2150-5594
http://hdl.handle.net/20.500.12105/9088
25531532
10.4161/21505594.2014.986412
2150-5608
Virulence
Cryptococcus neoformans is an encapsulated opportunistic fungal pathogen that is found in multiple niches in the environment and that can cause fatal meningoencephalitis in susceptible patients, mainly HIV+ individuals. Cryptococcus also infects environmental hosts such as nematodes, insects and plants. In particular, C. neoformans can kill the lepidopteran Galleria mellonella, which offers a useful tool to study microbial virulence and drug efficacy. Galleria mellonella immunity relies on innate responses based on melanization, accumulation of antimicrobial peptides, and cellular responses as phagocytosis or multicellular encapsulation. In this work we have investigated the immune response of G. mellonella during cryptococcal infection. We found that G. mellonella infected with C. neoformans had a high lytic activity in their hemolymph. This response was temperature- and capsule-dependent. During interaction with phagocytic cells, C. neoformans behaved as an intracellular pathogen since it could replicate within hemocytes. Non-lytic events were also observed. In contrast to Candida species, C. neoformans did not induce melanization of G. mellonella after infection. Finally, passage of C. neoformans through G. mellonella resulted in changes in capsule structure as it has been also reported during infection in mammals. Our results highlight that G. mellonella is an optimal model to investigate innate immune responses against C. neoformans.
eng
Cryptococcus neoformans
Galleria mellonella
Antimicrobial peptides
Hemocytes
Intracellular pathogenesis
Melanin
Cryptococcus neoformans induces antimicrobial responses and behaves as a facultative intracellular pathogen in the non mammalian model Galleria mellonella
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9088/1/CryptococcusNeoformansInducesAntimicrobial_2015.pdf
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CryptococcusNeoformansInducesAntimicrobial_2015.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/9088/3/CryptococcusNeoformansInducesAntimicrobial_2015.pdf.txt
File
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text/plain
CryptococcusNeoformansInducesAntimicrobial_2015.pdf.txt