2024-03-28T13:33:58Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/85302022-10-25T08:56:50Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Zaragoza, Oscar
author
Chrisman, Cara J
author
Castelli, Maria Victoria
author
Frases, Susana
author
Cuenca-Estrella, Manuel
author
Rodriguez-Tudela, Juan Luis
author
Casadevall, Arturo
funder
Ministerio de Educación y Ciencia (España)
funder
Instituto de Salud Carlos III
2019-10-25T10:04:47Z
2019-10-25T10:04:47Z
2008-10
Cell Microbiol. 2008 Oct;10(10):2043-57. doi: 10.1111/j.1462-5822.2008.01186.x. Epub 2008 Jun 28.
1462-5814
http://hdl.handle.net/20.500.12105/8530
18554313
10.1111/j.1462-5822.2008.01186.x
1462-5822
Cellular microbiology
Cryptococcus neoformans is a facultative intracellular pathogen. The most distinctive feature of C. neoformans is a polysaccharide capsule that enlarges depending on environmental stimuli. The mechanism by which C. neoformans avoids killing during phagocytosis is unknown. We hypothesized that capsule growth conferred resistance to microbicidal molecules produced by the host during infection, particularly during phagocytosis. We observed that capsule enlargement conferred resistance to reactive oxygen species produced by H(2)O(2) that was not associated with a higher catalase activity, suggesting a new function for the capsule as a scavenger of reactive oxidative intermediates. Soluble capsular polysaccharide protected C. neoformans and Saccharomyces cerevisiae from killing by H(2)O(2). Acapsular mutants had higher susceptibility to free radicals. Capsular polysaccharide acted as an antioxidant in the nitroblue tetrazolium (NBT) reduction coupled to beta-nicotinamide adenine dinucleotide (NADH)/phenazine methosulfate (PMS) assay. Capsule enlargement conferred resistance to antimicrobial peptides and the antifungal drug Amphotericin B. Interestingly, the capsule had no effect on susceptibility to azoles and increased susceptibility to fluconazole. Capsule enlargement reduced phagocytosis by environmental predators, although we also noticed that in this system, starvation of C. neoformans cells produced resistance to phagocytosis. Our results suggest that capsular enlargement is a mechanism that enhances C. neoformans survival when ingested by phagocytic cells.
eng
Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/8530/1/CapsuleEnlargementInCryptococcus_2008.pdf
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CapsuleEnlargementInCryptococcus_2008.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/8530/5/CapsuleEnlargementInCryptococcus_2008.pdf.txt
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CapsuleEnlargementInCryptococcus_2008.pdf.txt