2024-03-29T01:40:05Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/76982023-10-09T11:19:11Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Brasch, Melanie
author
Putri, Rindia M.
author
de Ruiter, Mark V.
author
Luque, Daniel
author
Koay, Melissa. S. T.
author
Castón, José R.
author
Cornelissen, Jeroen J. L. M.
funder
Comunidad de Madrid (España)
funder
Ministerio de Economía y Competitividad (España)
2019-05-30T10:45:30Z
2019-05-30T10:45:30Z
2017
J. Am. Chem. Soc.2017,139,4,1512-1519
0002-7863
http://hdl.handle.net/20.500.12105/7698
28055188
10.1021/jacs.6b10948
1520-5126
Journal of the American Chemical Society
The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as artificial nanocompartments for enzyme-catalyzed cascade reactions. Using the negative charges of nucleic acid tags, we develop a versatile strategy to promote an efficient noncovalent co-encapsulation of enzymes within a single protein cage of cowpea chlorotic mottle virus (CCMV) at neutral pH. The encapsulation results in stable 21-22 nm sized CCMV-like particles, which is characteristic of an icosahedral T = 1 symmetry. Cryo-EM reconstruction was used to demonstrate the structure of T = 1 assemblies templated by biological soft materials as well as the extra-swelling capacity of these T = 1 capsids. Furthermore, the specific sequence of the DNA tag is capable of operating as a secondary biocatalyst as well as bridging two enzymes for co-encapsulation in a single capsid while maintaining their enzymatic activity. Using CCMV-like particles to mimic nanocompartments can provide valuable insight on the role of biological compartments in enhancing metabolic efficiency.
eng
Assembling Enzymatic Cascade Pathways inside Virus-Based Nanocages Using Dual-Tasking Nucleic Acid Tags
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/7698/1/AssemblingEnzymaticCascadePathways_2017.pdf
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AssemblingEnzymaticCascadePathways_2017.pdf
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https://repisalud.isciii.es/bitstream/20.500.12105/7698/3/AssemblingEnzymaticCascadePathways_2017.pdf.txt
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AssemblingEnzymaticCascadePathways_2017.pdf.txt