2024-03-28T11:24:42Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/75092022-11-11T13:28:22Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2146
Repisalud
author
Sainz de Aja, Julio
author
Menchero, Sergio
author
Rollan, Isabel
author
Barral, Antonio
author
Tiana, Maria
author
Jawaid, Wajid
author
Cossio, Itziar
author
Alvarez, Alba
author
Carreño-Tarragona, Gonzalo
author
Badia-Careaga, Claudio
author
Nichols, Jennifer
author
Göttgens, Berthold
author
Isern, Joan
author
Manzanares, Miguel
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Wellcome Trust
funder
Fundación ProCNIC
2019-04-25T10:46:57Z
2019-04-25T10:46:57Z
2019-04-01
EMBO J. 2019; 38(7):e99122
0261-4189
http://hdl.handle.net/20.500.12105/7509
30814124
10.15252/embj.201899122
1460-2075
The EMBO journal
Progenitors of the first hematopoietic cells in the mouse arise in the early embryo from Brachyury-positive multipotent cells in the posterior-proximal region of the epiblast, but the mechanisms that specify primitive blood cells are still largely unknown. Pluripotency factors maintain uncommitted cells of the blastocyst and embryonic stem cells in the pluripotent state. However, little is known about the role played by these factors during later development, despite being expressed in the postimplantation epiblast. Using a dual transgene system for controlled expression at postimplantation stages, we found that Nanog blocks primitive hematopoiesis in the gastrulating embryo, resulting in a loss of red blood cells and downregulation of erythropoietic genes. Accordingly, Nanog-deficient embryonic stem cells are prone to erythropoietic differentiation. Moreover, Nanog expression in adults prevents the maturation of erythroid cells. By analysis of previous data for NANOG binding during stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct NANOG target. Our results show that Nanog regulates primitive hematopoiesis by directly repressing critical erythroid lineage specifiers.
eng
Nanog
Tal1
Erythropoiesis
Gastrulation
Primitive hematopoiesis
The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/7509/1/ThePluripotencyFactorNANOG_2019.pdf
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ThePluripotencyFactorNANOG_2019.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/7509/5/ThePluripotencyFactorNANOG_2019.pdf.txt
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ThePluripotencyFactorNANOG_2019.pdf.txt