2024-03-28T21:47:12Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/70262023-11-14T11:52:05Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Rubio-Lopez, Virginia
author
Valdezate, Sylvia
author
Álvarez, David
author
Villalon-Panzano, Pilar
author
Medina-Pascual, Maria Jose
author
Salcedo, Celia
author
Saez, Juan Antonio
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
2019-01-30T16:56:56Z
2019-01-30T16:56:56Z
2012-09-21
BMC Microbiol. 2012 Sep 21;12:215.
1471-2180
http://hdl.handle.net/20.500.12105/7026
22998619
10.1186/1471-2180-12-215
1471-2180
BMC microbiology
BACKGROUND: Group A Streptococcus (GAS) causes human diseases ranging in severity from uncomplicated pharyngitis to life-threatening necrotizing fasciitis and shows high rates of macrolide resistance in several countries. Our goal is to identify antimicrobial resistance in Spanish GAS isolates collected between 1994 and 2006 and to determine the molecular epidemiology (emm/T typing and PFGE) and resistance mechanisms of those resistant to erythromycin and tetracycline. RESULTS: Two hundred ninety-five out of 898 isolates (32.8%) were erythromycin resistant, with the predominance of emm4T4, emm75T25, and emm28T28, accounting the 67.1% of the 21 emm/T types. Spread of emm4T4, emm75T25 and emm28T28 resistant clones caused high rates of macrolide resistance. The distribution of the phenotypes was M (76.9%), cMLSB (20.3%), iMLSB (2.7%) with the involvement of the erythromycin resistance genes mef(A) (89.5%), msr(D) (81.7%), erm(B) (37.3%) and erm(A) (35.9%).Sixty-one isolates were tetracycline resistant, with the main representation of the emm77T28 among 20 emm/T types. To note, the combination of tet(M) and tet(O) tetracycline resistance genes were similar to tet(M) alone reaching values close to 40%. Resistance to both antibiotics was detected in 19 isolates of 7 emm/T types, being emm11T11 and the cMLSB phenotype the most frequent ones. erm(B) and tet(M) were present in almost all the strains, while erm(A), mef(A), msr(D) and tet(O) appeared in less than half of them. CONCLUSIONS: Spanish GAS were highly resistant to macrolides meanwhile showed minor resistance rate to tetracycline. A remarkable correlation between antimicrobial resistance and emm/T type was noticed. Clonal spread of emm4T4, emm75T25 and emm28T28 was the main responsable for macrolide resistance where as that emm77T28 clones were it to tetraclycline resistance. A wide variety of macrolide resistance genes were responsible for three macrolide resistance phenotypes.
eng
Molecular epidemiology, antimicrobial susceptibilities and resistance mechanisms of Streptococcus pyogenes isolates resistant to erythromycin and tetracycline in Spain (1994-2006)
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/7026/1/MolecularEpidemiologyAntimicrobialSusceptibilities_2012.pdf
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MolecularEpidemiologyAntimicrobialSusceptibilities_2012.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/7026/3/MolecularEpidemiologyAntimicrobialSusceptibilities_2012.pdf.txt
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MolecularEpidemiologyAntimicrobialSusceptibilities_2012.pdf.txt