2024-03-29T10:22:25Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/69622023-02-01T09:17:37Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16961col_20.500.12105_2061
Repisalud
author
Guardo, Alberto Crespo
author
Álvarez-Fernández, Carmen
author
Arberas, Hodei
author
García-Pérez, Javier
author
García, Felipe
author
Bargalló, Manuel Enric
author
Maleno, María José
author
Gatell, José María
author
Mothe, Beatriz
author
Alcamí, José
author
Sánchez-Palomino, Sonsoles
author
Plana, Montserrat
funder
Instituto de Salud Carlos III
funder
Government of Catalonia (España)
2018-12-27T11:09:14Z
2018-12-27T11:09:14Z
2013-03-14
PLoS One. 2013;8(3):e58927
1932-6203
http://hdl.handle.net/20.500.12105/6962
23516578
10.1371/journal.pone.0058927
1932-6203
PloS one
BACKGROUND: Generation of new reagents that can be used to screen or monitor HIV-1-specific responses constituted an interesting field in the development of HIV vaccines to improve their efficacy. METHODS: We have evaluated the specific T cell response against different types of NL4-3 virions (including NL4-3 aldrithiol-2 treated, NL4-3/ΔRT and R5 envelopes: NL4-3/ΔRT/ΔEnv[AC10] and NL4-3/ΔRT/ΔEnv[Bal]) and against pools of overlapping peptides (15 mer) encompassing the HIV-1 Gag and Nef regions. Cryopreserved PBMC from a subset of 69 chronic asymptomatic HIV positive individuals have been employed using different techniques including IFN-γ ELISPOT assay, surface activation markers and intracellular cytokine staining (ICS) by flow cytometry. RESULTS: The differential response obtained against NL4-3 aldrithiol-2 treated and NL4-3/ΔRT virions (25% vs 55%, respectively) allow us to divide the population in three groups: "full-responders" (positive response against both viral particles), "partial-responders" (positive response only against NL4-3/ΔRT virions) and "non-responders" (negative responses). There was no difference between X4 and R5 envelopes. The magnitude of the total responses was higher against NL4-3/ΔRT and was positively correlated with gender and inverse correlated with viral load. On the contrary CD4+ T cell count was not associated with this response. In any case responses to the viruses tended to be lower in magnitude than those detected by the overlapping peptides tested. Finally we have found an increased frequency of HLA-B27 allele (23% vs 9%) and a significant reduction in some activation markers (CD69 and CD38) on T cells surface in responders vs non-responders individuals. CONCLUSIONS: In summary these virions could be considered as alternative and useful reagents for screening HIV-1-specific T cell responses in HIV exposed uninfected people, HIV infected patients and to assess immunogenicity of new prototypes both in vitro and in vaccine trials, by a feasible, simply, effective and low cost assay.
eng
Use of RT-defective HIV virions: new tool to evaluate specific response in chronic asymptomatic HIV-infected individuals
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/6962/1/UseOfRT-DefectiveHIV_2013.pdf
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/6962/3/UseOfRT-DefectiveHIV_2013.pdf.txt
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UseOfRT-DefectiveHIV_2013.pdf.txt