2024-03-29T09:37:06Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/48552022-12-01T17:58:00Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Remoli, Maria Elena
author
Jimenez, Maribel
author
Fortuna, Claudia
author
Benedetti, Eleonora
author
Marchi, Antonella
author
Genovese, Domenico
author
Gramiccia, Marina
author
Molina, Ricardo
author
Ciufolini, Maria Grazia
2017-09-04T16:35:16Z
2017-09-04T16:35:16Z
2016-04-13
Parasit Vectors. 2016; 9: 205
http://hdl.handle.net/20.500.12105/4855
27075742
10.1186/s13071-016-1488-3
1756-3305
Parasites & Vectors
Phlebotomus-borne (PhB-) viruses are distributed in large areas of the Old World and are widespread throughout the Mediterranean basin, where recent investigations have indicated that virus diversity is higher than initially suspected. Some of these viruses are causes of meningitis, encephalitis and febrile illnesses. In order to monitor the viral presence and the infection rate of PhB-viruses in a recently identified and well characterized human zoonotic leishmaniasis focus in southwestern Madrid, Spain, a sand fly collection was carried out.
Methods
Sand fly insects were collected in four stations using CDC light traps during 2012–2013 summer seasons. Screening for Phlebovirus presence both via isolation on Vero cells and via polymerase chain reaction (PCR), using degenerated primers targeting a portion of the L segment, was performed. The serological identity and phylogenetic relationships on the three genomic segments of the viral isolates were carried out.
Results
Six viral isolates belonging to different serological complexes of the genus Phlebovirus were obtained from fifty pools on a total of 963 P. perniciosus (202 females). Phylogenetic analysis and serological assays allowed the identification of two isolates of Toscana virus (TOSV) B genotype, three isolates strongly related to Italian Arbia virus (ARBV), and one isolate of a novel putative Phlebovirus related to the recently characterized Arrabida virus in South Portugal, tentatively named Arrabida-like virus. Positive male sand fly pools suggested that transovarial or venereal transmission could occur under natural conditions.
Conclusions
Our findings highlighted the presence of different Phlebovirus species in the South-West area of the Madrid Autonomous Community where an outbreak of cutaneous and visceral human leishmaniasis has been recently described. The evidence of viral species never identified before in Spain, as ARBV and Arrabida-like virus, and TOSV B genotype focus stability was demonstrated. Environmental aspects such as climate change, growing urbanization, socio-economic development could have contributed to the genesis of this wide ecological niche of PhB-viruses and Leishmania spp. The potential role of vertebrates as reservoir for the phleboviruses identified and the possibility of Phleboviruses-Leishmania co-infection in the same sand fly should be assessed. Furthermore the PhB-viruses impact on human health should be implemented.
eng
Phlebovirus
Phlebotomus perniciosus
Toscana virus
Human leishmaniasis
Arbia virus
Novel virus
Madrid
Spain
Phleboviruses detection in Phlebotomus perniciosus from a human leishmaniasis focus in South-West Madrid region, Spain
journal article
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4855/1/PhlebovirusesDetectionInPhlebotomus_2016
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MD5
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PhlebovirusesDetectionInPhlebotomus_2016
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4855/14/13071_2016_Article_1488.pdf.txt
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13071_2016_Article_1488.pdf.txt
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https://repisalud.isciii.es/bitstream/20.500.12105/4855/16/PhlebovirusesDetectionInPhlebotomus_2016.txt
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PhlebovirusesDetectionInPhlebotomus_2016.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4855/22/PhlebovirusesDetectionInPhlebotomus_2016.txt
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PhlebovirusesDetectionInPhlebotomus_2016.txt