2024-03-28T10:12:12Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/47952022-10-06T07:09:31Zcom_20.500.12105_2074com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2075
Repisalud
author
Cordon-Obras, Carlos
author
Ochando, Jordi
author
Knapp, Jenny
author
Nebreda, Paloma
author
Ndong-Mabale, Nicolas
author
Ncogo-Ada, Policarpo Ricardo
author
Ndongo-Asumu, Pedro
author
Navarro, Miguel
author
Pinto, Joao
author
Benito, Agustin
author
Bart, Jean Mathieu
funder
Instituto de Salud Carlos III
2017-09-04T16:25:38Z
2017-09-04T16:25:38Z
2014-01-17
Parasit Vectors. 2014; 7: 31
http://hdl.handle.net/20.500.12105/4795
24438585
10.1186/1756-3305-7-31
1756-3305
Parasites & Vectors
BACKGROUND:
Luba is one of the four historical foci of Human African Trypanosomiasis (HAT) on Bioko Island, in Equatorial Guinea. Although no human cases have been detected since 1995, T. b. gambiense was recently observed in the vector Glossina palpalis palpalis. The existence of cryptic species within this vector taxon has been previously suggested, although no data are available regarding the evolutionary history of tsetse flies populations in Bioko.
METHODS:
A phylogenetic analysis of 60 G. p. palpalis from Luba was performed sequencing three mitochondrial (COI, ND2 and 16S) and one nuclear (rDNA-ITS1) DNA markers. Phylogeny reconstruction was performed by Distance Based, Maximum Likelihood and Bayesian Inference methods.
RESULTS:
The COI and ND2 mitochondrial genes were concatenated and revealed 10 closely related haplotypes with a dominant one found in 61.1% of the flies. The sequence homology of the other 9 haplotypes compared to the former ranged from 99.6 to 99.9%. Phylogenetic analysis clearly clustered all island samples with flies coming from the Western African Clade (WAC), and separated from the flies belonging to the Central Africa Clade (CAC), including samples from Mbini and Kogo, two foci of mainland Equatorial Guinea. Consistent with mitochondrial data, analysis of the microsatellite motif present in the ITS1 sequence exhibited two closely related genotypes, clearly divergent from the genotypes previously identified in Mbini and Kogo.
CONCLUSIONS:
We report herein that tsetse flies populations circulating in Equatorial Guinea are composed of two allopatric subspecies, one insular and the other continental. The presence of these two G. p. palpalis cryptic taxa in Equatorial Guinea should be taken into account to accurately manage vector control strategy, in a country where trypanosomiasis transmission is controlled but not definitively eliminated yet.
eng
Glossina palpalis palpalis
Luba
Equatorial Guinea
Tsetse
Trypanosomiasis
Phylogeny
Bioko
Glossina palpalis palpalis populations from Equatorial Guinea belong to distinct allopatric clades
journal article
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4795/1/GlossinaPalpalisPalpalisPopulations_2014.pdf
File
MD5
0644948e23e65fdc85fd3107c8eea45b
1252319
application/pdf
GlossinaPalpalisPalpalisPopulations_2014.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4795/20/13071_2013_Article_1179.pdf.txt
File
MD5
85c60e9a63540f9483cd6e3e1b7ab3e9
49644
text/plain
13071_2013_Article_1179.pdf.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4795/22/GlossinaPalpalisPalpalisPopulations_2014.pdf.txt
File
MD5
85c60e9a63540f9483cd6e3e1b7ab3e9
49644
text/plain
GlossinaPalpalisPalpalisPopulations_2014.pdf.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/4795/26/GlossinaPalpalisPalpalisPopulations_2014.pdf.txt
File
MD5
6b95925919bf1ce677a35136f550b6c7
49629
text/plain
GlossinaPalpalisPalpalisPopulations_2014.pdf.txt