2024-03-29T14:17:22Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/156772023-11-21T15:47:00Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2146
Repisalud
author
Izquierdo-Serrano, Raúl
author
Fernández-Delgado, Irene
author
Moreno-Gonzalo, Olga
author
Martín-Gayo, Enrique
author
Calzada-Fraile, Diego
author
Ramírez-Huesca, Marta
author
Jorge, Inmaculada
author
Camafeita, Emilio
author
Abián, Joaquín
author
Vicente-Manzanares, Miguel
author
Veiga, Esteban
author
Vazquez, Jesus
author
Sánchez-Madrid, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación Ramón Areces
funder
Fundación La Caixa
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares)
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2023-03-17T15:08:06Z
2023-03-17T15:08:06Z
2022
Front Immuno. 2022 Sep 5;13:946358
http://hdl.handle.net/20.500.12105/15677
36131943
10.3389/fimmu.2022.946358
1664-3224
Frontiers in immunology
Communication through cell-cell contacts and extracellular vesicles (EVs) enables immune cells to coordinate their responses against diverse types of pathogens. The function exerted by EVs in this context depends on the proteins and nucleic acids loaded into EVs, which elicit specific responses involved in the resolution of infection. Several mechanisms control protein and nucleic acid loading into EVs; in this regard, acetylation has been described as a mechanism of cellular retention during protein sorting to exosomes. HDAC6 is a deacetylase involved in the control of cytoskeleton trafficking, organelle polarity and cell migration, defense against Listeria monocytogenes (Lm) infection and other immune related functions. Here, we show that the protein content of dendritic cells (DCs) and their secreted EVs (DEVs) vary during Lm infection, is enriched in proteins related to antiviral functions compared to non-infected cells and depends on HDAC6 expression. Analyses of the post-translational modifications revealed an alteration of the acetylation and ubiquitination profiles upon Lm infection both in DC lysates and DEVs. Functionally, EVs derived from infected DCs upregulate anti-pathogenic genes (e.g. inflammatory cytokines) in recipient immature DCs, which translated into protection from subsequent infection with vaccinia virus. Interestingly, absence of Listeriolysin O in Lm prevents DEVs from inducing this anti-viral state. In summary, these data underscore a new mechanism of communication between bacteria-infected DC during infection as they alert neighboring, uninfected DCs to promote antiviral responses.
eng
Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/15677/1/Extracellular%20vesicles%20from%20Listeria%20Front%20Immuno%202022.pdf
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MD5
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application/pdf
Extracellular vesicles from Listeria Front Immuno 2022.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/15677/4/Extracellular%20vesicles%20from%20Listeria%20Front%20Immuno%202022.pdf.txt
File
MD5
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text/plain
Extracellular vesicles from Listeria Front Immuno 2022.pdf.txt